Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 23,191 results for: his

Clear keywords
Showing: 11321 - 11360 of 23191 results

  2. RGD-ACP-I27-CC

    Plasmid
    #85391
    Purpose
    MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and ACP tag for fluorophore labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to acyl carrier protein (ACP) tag and titin immunoglobulin domain (I27)
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85391-plasmid-map-sequence-id-172701

    Enlarge

  5. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid
    #101235
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    F491A, M495A, T496A, N497A and Q926A
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101235-plasmid-map-sequence-id-198037

    Enlarge

  6. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid
    #101234
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    T657A, G658A, W659A, R661A and Q926A
    Promoter
    T7
    Available Since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101234-plasmid-map-sequence-id-198016

    Enlarge

  7. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid
    #101220
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N692A, M694A, Q695A, H698A and Q926A
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101220-plasmid-map-sequence-id-197535

    Enlarge

  17. pJ131

    Plasmid
    #29485
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  34. TEVEp_pRK793

    Plasmid
    #247672
    Purpose
    pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVEp insert based on Yi et al., 2013.
    Depositor
    Carl Denard
    Article
    Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2
    Insert
    MBP-ENLYFES-7xHIS-TEVEp
    Tags
    His and MBP
    Expression
    Bacterial
    Mutation
    S120R, D148R, T173A, N177K, M218I, S219P
    Available Since
    Feb. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    247672-plasmid-map-sequence-id-491563

    Enlarge

  36. pTR01F/hFGFR3c-N540K-V5

    Plasmid
    #246390
    Purpose
    for PiggyBac mediated integration and stable expression of hFGFR3c-N540K protein, mutant variant causes Hypochondroplasia and Thanatophoric dysplasia
    Depositor
    Pavel Krejčí
    Article
    Ursachi et al J Bone Miner Res. 2026 Jan 26:zjag017. d
    Insert
    human FGFR3c-N540K (FGFR3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    N540K substitution
    Promoter
    truncated CMV promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246390-plasmid-map-sequence-id-491100

    Enlarge

  40. MZB131

    Plasmid
    #217839
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    AP3D1 (AP3D1 Human)
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Deleted residues 1-919
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217839-plasmid-map-sequence-id-432995

    Enlarge

Showing: 11321 - 11360 of 23191 results