We narrowed to 15,732 results for: LEA

1. pSBK.134

   Plasmid

   #187219

   Purpose

   Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectively

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Insert

   A: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   A: pTet*; B: pBetI

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-TEV-TBP6.9F34MF37MF77M

   Plasmid

   #168268

   Purpose

   Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   TBP6.9
   

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F34M, F37M and F77M

   Promoter

   T7 promotor

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2xFKBP-mCh-KDEL

   Plasmid

   #173010

   Purpose

   FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   2xFKBP-mCh-KDEL
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEf1a OsTIR ires NEO pA T2BH

   Plasmid

   #127910

   Purpose

   Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   OsTIR
   

   Use

   Transposon

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pclbw-eGFPomp25

   Plasmid

   #58424

   Purpose

   Mammalian expression of GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   eGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   CMV/b-actin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-hSyn-GRAB_g5-HT2h

   Plasmid

   #208721

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT2h
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6GRNA

   Plasmid

   #68370

   Purpose

   gRNA scaffold with hU6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCl-HA-YAP

   Plasmid

   #27007

   Depositor

   Joan Massague

   Article

   Alarcon et al Cell. 2009 Nov 13. 139(4):757-69.

   Insert

   YAP (YAP1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-N3-TPC2

   Plasmid

   #80153

   Purpose

   Intracellular Ca2+ store marker

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

   Insert

   TPCN2 (TPCN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-CUL1 delta 610-615

    Plasmid

    #19941

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 delta 610-615 (CUL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CUL1 with a ROC1 binding mutant

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNAintron-CHIKV-capsid protein

    Plasmid

    #215701

    Purpose

    Produces the Chikungunya virus capsid protein

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV capsid
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3-HA-CUL1 delta C22

    Plasmid

    #19942

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 delta C22 (CUL1 Human)
    

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutation of 22 amino acids from the C terminus

    Available Since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pcDNAintron-CDC50A-c3xFLAG

    Plasmid

    #215702

    Purpose

    Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CDC50A (TMEM30A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pQCXIX_sh_HOIP/RNF31_#6

    Plasmid

    #75252

    Purpose

    retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against human RNF31 (RNF31 )
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABEPuro-HA-GLI1

    Plasmid

    #62967

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. VRQR variant

    Plasmid

    #138562

    Purpose

    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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