We narrowed to 5,779 results for: chia
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Plasmid#236224PurposeExpress N-terminal His-tagged human SMSr (SAMD8) protein in insect cellsDepositorInsertSAMD8 (SAMD8 Human)
TagsHexa histidine tag (6xHis-tag) and enterokinase s…ExpressionInsectMutationwild typeAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdnCas3-CDA1
Plasmid#229535PurposepMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsdnCas3
Cytidine deaminase
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdnCas3-APOBEC1
Plasmid#229537PurposepMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
dnCas3
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+Strep-Opa1 (SB254)
Plasmid#227603PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastorisDepositorInsertsTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[R247A,R347A]-His (SB257)
Plasmid#227606PurposeInducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1(MGD) (SB252)
Plasmid#227601PurposeInducible expression of Strep-tagged Opa1(MGD) in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
TagsTwin-StrepTagExpressionYeastMutationcontains aa 253-580 (MGD) and aa 938-960PromoterAOX1Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-CD28 CAR hinge (I114 – P152)
Plasmid#224929PurposeExpression GST fusion CD28 CAR hinge (I114-P152) in Escherichia coli.DepositorInsertCD28 CAR hinge (I114 – P152) (TMIGD2 Human)
Tagsglutathione-S-transferaseExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1-lifeact-oROS-HT
Plasmid#216420PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.DepositorInsertlifeact-oROS-HT
ExpressionMammalianPromoterCMVAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T]
Plasmid#60753PurposeContains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.DepositorInsertsGalS-T
GalS-T
UseSynthetic BiologyExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…Available SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-rtTAV16
Plasmid#102367PurposeAAV vector to drive TET-ON activator expression from human synapsin I promoterDepositorInsertrtTAV16
UseAAVExpressionMammalianMutationrtTA variant reported in Gene Therapy (2006) 13, …Promoterhuman synapsin I promoterAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon
ExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-CMV-mCherry-1xU6-LtR-wt(TAG)
Plasmid#217366PurposeExpresses E. coli leucine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli leucine tRNA for TAG suppression
UseAAVExpressionMammalianPromoterU6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
SPACE (pRZ1813)
Plasmid#140242PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE
ExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcBac2.tR4-OMeYRS/GFP*
Plasmid#50831Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Tyr tRNA cassette
EGFP*
OMeYRS
two copy Tyr tRNA cassette
UseBaculoviralTagsHis, Myc-6xHis, and WPREExpressionInsectMutationY39TAG and able to charge various unnatural amino…PromoterCAG, CMV, and U6 and H1Available SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE_NG
ExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMG024 MBP-GSK3β-HA-His, GST_λPPase
Plasmid#196182PurposeCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-HisDepositorAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
Plasmid#176534PurposeBacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrepDepositorInsertCRISPR-associated IscB (Delaware Bay metagenome)
Tags14xHis-MBP-bdSUMO and TwinStrepExpressionBacterialPromoterT7Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-LacI
Plasmid#103814PurposeBLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only