We narrowed to 15,685 results for: genome

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enAsCas12a-mSA

Plasmid

#182954

Purpose

Express enAsCas12a-mSA

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

enAsCas12a-mSA

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJG363

Plasmid

#91183

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gNt_R2+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG370

Plasmid

#91187

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gRNActrl+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pQCi-hTRAC-sgRNA

Plasmid

#154093

Purpose

pQCi backbone with sgRNA targeting human TCR-alpha common chain

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting human TRAC locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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SL2-sgSat-MTSb/BFP/pdCas9-C1

Plasmid

#162761

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting centromeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgSat-MTSb)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB2803

Plasmid

#63646

Purpose

pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9_1

Plasmid

#91156

Purpose

Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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CD200RCd4d3+4-blac

Plasmid

#32407

Purpose

A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Tags

Cd4d3+4 and beta-lactamase

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAYC

Plasmid

#62657

Purpose

Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

iCre

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_2

Plasmid

#91160

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pKS913.CB18.31

Plasmid

#69569

Purpose

TCR beta chain genomic transgenic construct for creating OT-1 mice

Depositor

Francis R. Carbone

Article

Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

Insert

TCR beta (Tcrb Mouse)

Use

Mouse Targeting

Expression

Bacterial

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBA1018

Plasmid

#189587

Purpose

T4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

T4wtGT7 Deletion Locus 250bp Homology Arms

Use

Synthetic Biology

Expression

Bacterial

Mutation

Encodes for a full deletion of T4 from 52.1 (part…

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pU6nH1-HD10kb

Plasmid

#172657

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD10kbA/pegRNA-HD10kbB

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pICSL80036

Plasmid

#68259

Purpose

Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_A0801

Plasmid

#91022

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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gh54

Plasmid

#106727

Purpose

expression of gRNA targeting ST3GAL6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST3GAL6 (ST3GAL6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCFL-mPML-3KRmut-iB

Plasmid

#140287

Purpose

Expression of mouse PML KR mutant

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

PML (Pml Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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