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FUW-KO

Plasmid

#20331

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-Oct4

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FAT FAK biosensor

Plasmid

#78303

Purpose

FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

Depositor

Yingxiao Wang

Publication

Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FAT FAK biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGWB514

Plasmid

#74856

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBabe.puro.CDK8.KD.flag

Plasmid

#19759

Depositor

William Hahn

Publication

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

D173A (kinase dead)

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only
GST HP1 alpha

Plasmid

#24074

Depositor

Naoko Tanese

Publication

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 alpha (CBX5 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA4-Beclin1 1-150

Plasmid

#24389

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 1-150.

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAAV-myrSNAP

Plasmid

#60098

Purpose

Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional

Depositor

Gregory Jefferis

Publication

Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

Inserts

SNAP-tag
histone-GFP

Use

AAV and Mouse Targeting

Expression

Mammalian

Available since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PML-3/pSG5

Plasmid

#50939

Purpose

expression of PML with Sumo sites destroyed

Depositor

Pier Pandolfi

Publication

Zhong et al Blood. 2000 May 1;95(9):2748-52.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PML-3 (PML Human)

Tags

HA

Expression

Mammalian

Mutation

K65R, K160R, K490R (Sumo modification sites)

Promoter

SV40

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Publication

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDK4

Plasmid

#116724

Purpose

Lentiviral expression of CDK4

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCI-Neo-gp78/JM20

Plasmid

#13303

Depositor

Allan Weissman

Publication

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gp78 (AMFR Human)

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pGWB535

Plasmid

#74873

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB433

Plasmid

#74824

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEF6-Acly(S455D)

Plasmid

#70768

Purpose

Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Publication

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Mutation

S455D

Promoter

EF1alpha

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Publication

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB511

Plasmid

#74853

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB404

Plasmid

#74798

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-LEU2MX6

Plasmid

#33138

Purpose

gene deletion in S. cerevisiae and S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Insert

LEU2MX6

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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