We narrowed to 17,102 results for: EMB

1. EasyFusion T2A-H2B-miRFP703

   Plasmid

   #113097

   Purpose

   General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-miRFP703
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-miniTurbo-Puro

   Plasmid

   #207725

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TOPO mKate2

   Plasmid

   #68441

   Purpose

   TOPO construct expressing mKate2 for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mKate2
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-300(HAT-)

   Plasmid

   #23254

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Expression

   Mammalian

   Mutation

   H1415A E1423A Y1424A L1428S Y1430A H1434A

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-GFP-Sec61β

   Plasmid

   #172442

   Purpose

   Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Protein transport protein Sec61 subunit beta
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HH-gRNA-HDV-Shuttle-Vector

   Plasmid

   #169098

   Purpose

   Backbone plasmid to generate HH-gRNA-HDV segment

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Development. 2021 Mar 9. pii: dev.193565

   Insert

   HH-gRNAbackbone-HDV
   

   Expression

   Mammalian

   Promoter

   NA

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-SauCas9

   Plasmid

   #174040

   Purpose

   In vitro transcription of SauCas9 mRNA

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   SauCas9
   

   Use

   In vitro transcription

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-NHERF1 WT

   Plasmid

   #195213

   Purpose

   Mammalian expression vector for GFP tagged NHERF1.

   Depositor

   Anthony Bretscher, David Bryant

   Article

   Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

   Insert

   NHERF1 (NHERF1 Human)
   

   Expression

   Mammalian

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTB3-BC2 nanobody

   Plasmid

   #210208

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   BC2 Nanobody
   

   Expression

   Bacterial

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHRSin_hTCRβ

    Plasmid

    #187361

    Purpose

    Lentiviral expression of GPa3b17 hTCR beta

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    GPa3b17 hTCR beta
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHRSin_hTCRα

    Plasmid

    #187360

    Purpose

    Lentiviral expression of GPa3b17 hTCR alpha

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    GPa3b17 hTCR alpha
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCMU-PDESr

    Plasmid

    #61206

    Purpose

    Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtPDLP1
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-R-iGluSnFR1

    Plasmid

    #107335

    Purpose

    Red fluorescent glutamate sensor based on cpmApple. Membrane-displayed

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    R-iGluSnFR1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRc/CMV HA TrkB

    Plasmid

    #39978

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkB (Ntrk2 Rat)
    

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    endogenous signal sequence was replaced with BM40…

    Promoter

    CMV

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. EasyFusion T2A-H2B-TagBFP

    Plasmid

    #113086

    Purpose

    General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-TagBFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDR119

    Plasmid

    #13332

    Depositor

    Martin Gellert

    Article

    Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

    Insert

    DNA ligase IV (LIG4 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pDEST14-DoubleCatcher

    Plasmid

    #216284

    Purpose

    Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3_PKA-RIIα-sREACh

    Plasmid

    #168489

    Purpose

    Mammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    PKA regulatory subunit IIα C-terminally tagged by sREACh
    

    Expression

    Mammalian

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p006-Banana-Late

    Plasmid

    #112251

    Purpose

    ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    ATF1 under osmY promoter
    

    Expression

    Bacterial

    Promoter

    osmY

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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