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pEGFP Tubulin K40R

Plasmid

#105303

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40R

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 WT

Plasmid

#195213

Purpose

Mammalian expression vector for GFP tagged NHERF1.

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMK484 (2mAID-Hygro)

Plasmid

#214396

Purpose

2mAID for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

2mAID-Hygro

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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IL13 promoter luciferase

Plasmid

#11784

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-13 promoter (IL13 Human)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only
p005-RFP-strong

Plasmid

#108317

Purpose

Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of strong synthetic constitutive promoter pFAB4005

Expression

Bacterial

Promoter

pFAB4005

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p006-Banana-Late

Plasmid

#112251

Purpose

ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

ATF1 under osmY promoter

Expression

Bacterial

Promoter

osmY

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p1242 3x-KB-L

Plasmid

#26699

Depositor

Bill Sugden

Article

Mitchell et al J Virol. 1995 May . 69(5):2968-76.

Insert

3x MHC I KB element - luciferase (NFKB1 Human, Photinus pyralis)

Use

Luciferase

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1

Plasmid

#67900

Purpose

Expresses a fusion of mCherry with FKBP12 in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mNeon

Plasmid

#207687

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Article

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-PGK-Puro

Plasmid

#207742

Purpose

Co-expression of the Cre recombinase and a puro selectable marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-Puro

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-3xFLAG

Plasmid

#207695

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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NES-EGFP-P4Mx1

Plasmid

#108121

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS2699-CMV-Nme2Cas9-E932R

Plasmid

#228720

Purpose

Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2696-CMV-Nme2SmuCas9-D56R

Plasmid

#228717

Purpose

Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-D56R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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