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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-L3F6H

Plasmid

#177765

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCLXSN-c-JUN

Plasmid

#102758

Purpose

Expression of c-Jun

Depositor

Jin Chen

Article

Song et al Cancer Res. 2014 May 1;74(9):2444-54. do

Insert

c-Jun (JUN Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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LV-miR-142

Plasmid

#117056

Purpose

Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

miR-142-3p - OFP (Mir142 Mouse)

Use

Lentiviral

Promoter

SFFV promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CREB3L1

Plasmid

#214767

Purpose

Mammalian expression of human CREB3L1

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

CREB3L1 (CREB3L1 Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCOCK CMVwt

Plasmid

#46559

Purpose

Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.

Depositor

Clifford Wang

Article

Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

Insert

CMV wt promoter

Use

Synthetic Biology

Expression

Mammalian

Mutation

Our version has a G at base 724 (reference has C)

Promoter

CMV

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 KD, dominant negative chk2

Plasmid

#41902

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D347A

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K370A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-Zc3h12a 3’UTR

Plasmid

#222662

Purpose

Luciferase reporter vector containing mouse Zc3h12a 3'UTR

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a 3'UTR (Zc3h12a Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

Plasmid

#184395

Purpose

Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-3xFLAG-YAP1-S127A

Plasmid

#42239

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3xFLAG

Mutation

Serine 127 changed to alanine

Promoter

none

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Grhl3

Plasmid

#172869

Purpose

Mammalian expression vector with myc-tagged mouse Grhl3 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Grhl3 (Grhl3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Cox7c-pHLuorin-sems

Plasmid

#107766

Purpose

labeling of mitochondrial cytochrome c oxidase, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit Cox7c fused to pHluorin (COX7C Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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139H2_HC

Plasmid

#206201

Purpose

For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

Depositor

Joost Snijder

Article

Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

Insert

anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)

Tags

-AAAHHHHHHHH

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-eIF6-3XFlag

Plasmid

#242188

Purpose

Express eIF6-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

eIF6 (EIF6 Human)

Use

Lentiviral

Tags

3xFlag

Promoter

CMV

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-p62(ΔUBD)

Plasmid

#119687

Purpose

For mammalian expression of p62 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

UBD deletion (1-385)

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

Plasmid

#212675

Purpose

Express tagged hRb phosphosite mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

15 CDK phospho-sites are mutated to alanines, and…

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

Plasmid

#184396

Purpose

Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291A

Plasmid

#159160

Purpose

Expresses DEPTOR S286/287/291A mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to alanine

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291D

Plasmid

#159161

Purpose

Expresses DEPTOR S286/287/291D mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to aspartic acid

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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