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pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

Plasmid

#197454

Purpose

The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 Delta-WD40 propeller (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW& dimerization domain deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1-861-dsRNAres_R

Plasmid

#147401

Purpose

Insect Expression of DmGW182_1-861-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_1-861-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182_1-861-dsRNAres_R

Plasmid

#147408

Purpose

Insect Expression of DmGW182_1-861-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_1-861-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-12xmut_1-539_O

Plasmid

#147157

Purpose

Insect Expression of DmGW182-12xmut_1-539

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-12xmut_1-539 (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182_1-830-dsRNAres_G

Plasmid

#146388

Purpose

Insect Expression of DmGW182_1-830-dsRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182_1-830-dsRNAres (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182_1-1115-dsRNAres_G

Plasmid

#146389

Purpose

Insect Expression of DmGW182_1-1115-dsRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182_1-1115-dsRNAres (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182_1-605-dsRNAres_G

Plasmid

#146387

Purpose

Insect Expression of DmGW182_1-605-dsRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182_1-605-dsRNAres (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Djun - antisense

Plasmid

#38338

Depositor

Norbert Perrimon

Article

Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

Insert

djun (Jra Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
HIV-1-GFP ΔEnv

Plasmid

#217437

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (N154S)

Plasmid

#214160

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (N154S) (STING1 Human)

Expression

Mammalian

Mutation

N154S

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2_C277S-Flag

Plasmid

#180412

Purpose

lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2_C277S (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C277S

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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GZnP2 in pcDNA3.1+

Plasmid

#161739

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Article

Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

Insert

GZnP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL7-pCMV7.1

Plasmid

#155025

Purpose

N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 7 (CUL7 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LARG mCherry sspB in pcDNA3.1

Plasmid

#90459

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mCherry
sspB

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-TN-DROSHA

Plasmid

#106252

Purpose

Express TN-DROSHA in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

Insert

Drosha Ribonuclease III (DROSHA Human)

Tags

V5-His

Expression

Mammalian

Mutation

E1045Q and E1222Q mutations

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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plko.1/shMCT4 #474

Plasmid

#99597

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-CMV 7.1_syn6

Plasmid

#50012

Purpose

Encodes syntaxin 6 with a N-terminal 3X FLAG tag

Depositor

David Hackstadt

Article

Moore et al Microbiology. 2011 Mar;157(Pt 3):830-8.

Insert

syntaxin 6 (Stx6 Rat)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_FLAG-pcDNA 3.1

Plasmid

#73593

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA Oct4A -12

Plasmid

#50921

Purpose

U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Oct4A -12 sgRNA (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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