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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Article

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

Plasmid

#199346

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B3

Plasmid

#191668

Purpose

ddFP-B3-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddFP-B3

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

Plasmid

#167902

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLBS.EF NLS-pHlu

Plasmid

#129324

Purpose

pH measurement, nucleus, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

pHluorin (from H. Imamura)

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSM_ser-2prom3p::myr-mCherry

Plasmid

#164223

Purpose

Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

Depositor

Yan Zou

Article

Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

Insert

myr-mCherry

Expression

Worm

Promoter

ser-2promo3

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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MYH11-gRNA1

Plasmid

#132587

Purpose

gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

Depositor

James Thomson

Article

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

MYH11-gRNA1

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT2-Tbait-UAS-Positron-ST

Plasmid

#129266

Purpose

UAS-driven expression of Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP L138 gRNA

Plasmid

#119133

Purpose

gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L138 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG041

Plasmid

#113997

Purpose

Level 0 module. Taurine-inducible promoter for Alphaproteobacteria

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Taurine promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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AP568-3

Plasmid

#70048

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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AP568-2

Plasmid

#70047

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRibo EcoHind

Plasmid

#36252

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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AP-p002

Plasmid

#31493

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pAAV hSyn CaBLAM

Plasmid

#244227

Purpose

Bioluminescent reporter for calcium signaling in neurons

Depositor

Ute Hochgeschwender

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEHAC1-CHIP-GSGS-BirA-HA

Plasmid

#232588

Purpose

Express BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

CHIP - BirA (CHIP Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-184_Cacnes_mCherry

Plasmid

#225610

Purpose

Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

mCherry

Use

Synthetic Biology

Tags

V5-6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-trAtCCD8(pYL735)

Plasmid

#178286

Purpose

Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4531

Plasmid

#132366

Purpose

Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Tags

none

Expression

Worm

Promoter

Prgef-1

Available Since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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