We narrowed to 6,488 results for: cki

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Hiv7CMV-Cremyc-2A-GFP

Plasmid

#117148

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Use

Lentiviral

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCheck2 Dest (R1-R2) #BGV129

Plasmid

#48955

Purpose

Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Luciferase; Gateway cloning vector

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pLY239

Plasmid

#184152

Purpose

Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-R3

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY273

Plasmid

#184155

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of zraP

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-zraP-22

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-AD1-deletion

Plasmid

#75265

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 1

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

AD1 (activation domain 1) deleted

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-AD2-deletion

Plasmid

#75266

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 2

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

AD2 (activation domain 2) deleted

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dC

Plasmid

#176466

Purpose

express VPS13D lacking C-terminal 407 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

C-terminal 407 aa is deleted.

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CSNK2A1

Plasmid

#67085

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_delta ald1

Plasmid

#126708

Purpose

vector for knocking out ald1 in Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

HIS4

Expression

Yeast

Promoter

His

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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FRIG-myc-Sema4DdeltaC

Plasmid

#51607

Purpose

expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

deleted last 70 amino acids in the C-terminus

Promoter

RSV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-SpEAF 60-251 pBacPAK8

Plasmid

#15321

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

EAF1 (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Mutation

N terminal deletion lacking first 59 amino acids …

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pTKI-Tyr

Plasmid

#247647

Purpose

Expresses GFP-SUMO-Tyr-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1

Depositor

Karl Schmitz

Article

Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do

Insert

GFP-SUMO-Tyr-mCherry N-degron proteolysis dual-fluorescence reporter

Use

Mycobacterial integrating

Tags

HA tag and Myc tag

Expression

Bacterial

Promoter

PTet

Available Since

Dec. 9, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTKI-Ser

Plasmid

#247648

Purpose

Expresses GFP-SUMO-Ser-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1

Depositor

Karl Schmitz

Article

Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do

Insert

GFP-SUMO-Ser-mCherry N-degron proteolysis dual-fluorescence reporter

Use

Mycobacterial integrating

Tags

HA tag and Myc tag

Expression

Bacterial

Promoter

PTet

Available Since

Dec. 9, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HA-RIPLET ΔPRY/SPRY

Plasmid

#218516

Purpose

RIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1

Depositor

Sun Hur

Article

Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

Insert

RIPLET delta PRY/SPRY domain (RNF135 Human)

Expression

Mammalian

Available Since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

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Combined γB2ΔICD-FRET probe

Plasmid

#209093

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_CVB1_VP1

Plasmid

#202069

Purpose

Bacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB1 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_CVA4_VP1

Plasmid

#202068

Purpose

Bacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVA4 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T564A

Plasmid

#191978

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T564A (Mfn1 Mouse)

Expression

Mammalian

Mutation

T564A

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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