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pMSCV-puro-TTF-1-HDD

Plasmid

#74991

Purpose

Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 HDD mutant (NKX2-1 Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

HDD = homeodomain deleted

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-delta 158-180

Plasmid

#78923

Purpose

Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 159-180

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 189-215

Plasmid

#78924

Purpose

Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 189-215

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-MKLP1 S708/710A

Plasmid

#70153

Purpose

mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S708A, S710A

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARL1-V5-His6

Plasmid

#67437

Purpose

Mammalian expression of hArl1 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

His and V5

Expression

Mammalian

Mutation

bp 231 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pJH104

Plasmid

#48262

Purpose

Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.

Depositor

Ronald Davis

Article

Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

Inserts

TEF1p 5' fragment
URA3 5' fragment
TEF1p 3' fragment

Use

Routine cloning vector

Mutation

From SK1 background. -343 to -1 relative to TEF1 …

Promoter

TEF1p

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_V12-S35

Plasmid

#39517

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-K225insNM

Plasmid

#31974

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

inserted amino acids Asn-Met before Lys225

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP-Ofd1-Myc-A80T

Plasmid

#24579

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-DD-KNL1-rvsf_aaaa-dCas9

Plasmid

#248565

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

DD-KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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mILK-R255A/R349A-EFGP

Plasmid

#176899

Purpose

mouse ILK with R255A and R349A point mutations cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 255 to Alanine and Arginine 349 to Alani…

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173875

Purpose

Mammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173876

Purpose

Mammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140

Plasmid

#136601

Purpose

Lentiviral vector encoding mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140 under the EF1A promoter.

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-FRB-P2A-T2A-FKBP-C-mSOG

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-AKAR

Plasmid

#123329

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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Venus2-K49R-STAT3

Plasmid

#123167

Purpose

Expresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

K49R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Venus2-K140R-STAT3

Plasmid

#123169

Purpose

Expresses K140R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

K140R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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