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We narrowed to 5,779 results for: chia

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Showing: 5761 - 5779 of 5779 results

  1. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234441
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Has Service
    AAV1
    Insert
    iGluSnFR4s-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234441-plasmid-map-sequence-id-465749

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  2. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234448
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234448-plasmid-map-sequence-id-465743

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  3. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  4. pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234447
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234447-plasmid-map-sequence-id-465730

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  5. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234442
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Has Service
    AAV1
    Insert
    iGluSnFR4f-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234442-plasmid-map-sequence-id-465693

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  6. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234440
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234440-plasmid-map-sequence-id-465720

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  7. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234439
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234439-plasmid-map-sequence-id-465689

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  8. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234454
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234454-plasmid-map-sequence-id-465691

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  10. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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  12. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

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  13. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

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  14. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid
    #227609
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-544, 651-757 only [TM deleted]
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227609-plasmid-map-sequence-id-455572

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  15. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

    Enlarge

  16. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid
    #227610
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-400, 706-757 [TM and HB2 deleted]
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227610-plasmid-map-sequence-id-455569

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  17. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

    Enlarge

  18. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid
    #209086
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Tags
    AviTag
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available Since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209086-plasmid-map-sequence-id-417159

    Enlarge

  19. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid
    #209085
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available Since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    209085-plasmid-map-sequence-id-416546

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Showing: 5761 - 5779 of 5779 results