We narrowed to 2,455 results for: GEM

1. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

   Plasmid

   #176932

   Purpose

   Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-sgMUC1_dual-MTS-3x-Set I

   Plasmid

   #162763

   Purpose

   Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174840

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ENO1p

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-3XP3.RFP-tan

   Plasmid

   #83474

   Purpose

   RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   3XP3.RFP-tan
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   3XP3 eye-expression Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-mClover3-MAP4-MTBD

   Plasmid

   #171498

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   So et al Science. 2019 Jun 28;364(6447). pii: 364

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+ GEMIN6 myc HIS

   Plasmid

   #71659

   Purpose

   Mammalian expression of GEMIN6

   Depositor

   Gideon Dreyfuss

   Insert

   GEMIN6 (GEMIN6 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Rab27a cds in pGem-T Easy

   Plasmid

   #80546

   Purpose

   Can be used for in situ hybridisation or other purposes

   Depositor

   Rob Parton

   Article

   Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

   Insert

   rab27a (rab27a Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-mCherry-mTrim21ΔRING-Box

   Plasmid

   #105523

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Expression

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGem-PgpdA-Cre-TtrpC

   Plasmid

   #168784

   Purpose

   Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

   Insert

   Cre
   

   Use

   Cre/Lox and Synthetic Biology; Expression in fila…

   Tags

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid

    #191388

    Purpose

    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_hSLBP(56_108)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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