We narrowed to 8,769 results for: ef1

1. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

   Plasmid

   #120513

   Purpose

   Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC Thr58Ala (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Point mutation changing Threonine to Alanine at a…

   Promoter

   EF1a

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

   Plasmid

   #120506

   Purpose

   Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC H84NLS (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of nuclear localization signal sequence

   Promoter

   EF1a

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPL5628_TEF1*-Cre_MET15

   Plasmid

   #60933

   Purpose

   Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   mutant TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1_Cre

   Plasmid

   #201198

   Purpose

   AAV vector for cre expression under EF1 promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Inserts

   cre
   EF1alpha promoter
   

   Use

   AAV

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   nuclear targeting cre

   Promoter

   N/A and hEF1alpha promoter

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_EEF1A2_GTP-EFTU

   Plasmid

   #109862

   Purpose

   Protein expression and purification of EEF1A2_GTP-EFTU

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   EEF1A2_GTP-EFTU (EEF1A2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-mRor2N620K

   Plasmid

   #22631

   Depositor

   Roel Nusse

   Article

   Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

   Insert

   Ror2 N620K (Ror2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Asparagine 620 to Lysine

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pET15-EF1p_mFAP2b

   Plasmid

   #159469

   Purpose

   Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1p_mFAP2b
   

   Use

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDV05.1_pEF1a-iRFP720_frameshifted

   Plasmid

   #200408

   Purpose

   Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720 (frameshift mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15-EF1p3_mFAP2b

   Plasmid

   #159470

   Purpose

   Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1p3_mFAP2b
   

   Use

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-nSpCas9-NG

    Plasmid

    #171692

    Purpose

    Expresses nSpCas9-NG in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9-NG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

    Promoter

    EF1a

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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