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Showing: 561 - 580 of 1459 results

  1. His8:MBP-tev-Lys-eK-Flv

    Plasmid
    #83387
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83387-plasmid-map-sequence-id-175064

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  2. His8:MBP-tev-Leu-eK-Flv

    Plasmid
    #83386
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83386-plasmid-map-sequence-id-175060

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  3. His8:MBP-tev-Arg-eK-Flv

    Plasmid
    #83385
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83385-plasmid-map-sequence-id-173975

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  4. His8:MBP-tev-Phe-eK-Flv

    Plasmid
    #83384
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83384-plasmid-map-sequence-id-173972

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  7. pHR myc-MBP-SspB-iRFP-NLS

    Plasmid
    #223286
    Purpose
    An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.
    Depositor
    Jared Toettcher
    Article
    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860
    Insert
    Myc-MBP-SspB-irFP-NLS
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    223286-plasmid-map-sequence-id-442283

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  9. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Use
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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  10. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid
    #193051
    Purpose
    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    ALA7-TMD
    Use
    Tags
    Ig k-chain leader sequence, MBP, and mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193051-plasmid-map-sequence-id-386362

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  13. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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Showing: 561 - 580 of 1459 results