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We narrowed to 1,212 results for: Ncl

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Showing: 841 - 870 of 1212 results

  3. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  5. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T1
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97327-plasmid-map-sequence-id-182625

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  11. pAAV-hSyn-Gluc-feRMA-IRES-EGFP

    Plasmid
    #248190
    Purpose
    Expresses Gluc-feRMA and EGFP under the hSyn promoter. Gluc-feRMA enables monitoring of neuronal transduction and includes a TEV cs, allowing inactivation of it upon interaction with TEV protease.
    Depositor
    Jerzy Szablowski
    Article
    Nouraein et al bioRxiv 2025.05.08.652140
    Inserts
    Gaussia luciferase with TEV cleavage site fused to IgG1 Fc
    IRES-EGFP
    Use
    AAV; Luciferase
    Tags
    Gluc (N terminal on insert) and IgG1 Fc (C termin…
    Expression
    Mammalian
    Promoter
    IRES and hSyn
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248190-plasmid-map-sequence-id-495612

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  12. pLVX-TetOne-puro-atpG

    Plasmid
    #248067
    Purpose
    Lentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpG (atpG E. coli)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248067-plasmid-map-sequence-id-493799

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  13. pLVX-TetOne-bsd-atpA

    Plasmid
    #248065
    Purpose
    Lentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpA
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248065-plasmid-map-sequence-id-493789

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  14. pPB-CrPV-IRES-cytERM-SUNTAG-MS2

    Plasmid
    #247299
    Purpose
    Mammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation.
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-
    Insert
    PB-CrPV-IRES-cytERM-SUNTAG-MS2
    Expression
    Mammalian
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    247299-plasmid-map-sequence-id-491542

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  15. pAAV-hsyn-DIO-AppNL-G-F-T2A-mCherry

    Plasmid
    #242649
    Purpose
    Floxed (DIO) AAV transgene designed to be packaged in AAV and to express the amyloid precursor protein (App) with familial mutations AppNL-G-F in a Cre-recombinase-dependent manner.
    Depositor
    William Wisden
    Insert
    Floxed (DOI) AAV transgene expressing an amyloid precursor protein with familial mutations and an mCherry tag
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Mutation
    The amyloid precursor reading frame contains a c…
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    242649-plasmid-map-sequence-id-489352

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  16. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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  17. PC2328

    Plasmid
    #232089
    Purpose
    Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.
    Depositor
    Pranam Chatterjee
    Article
    Hong et al Nat Commun. 2025 Apr 15;16(1):3555. doi:
    Insert
    duAb with cloning Esp3I restriction site
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232089-plasmid-map-sequence-id-469903

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  18. pDM043

    Plasmid
    #216813
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216813-plasmid-map-sequence-id-430146

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  19. pDM052

    Plasmid
    #216814
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216814-plasmid-map-sequence-id-429539

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  20. pDM070

    Plasmid
    #216816
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216816-plasmid-map-sequence-id-429610

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  22. pTS2343_Tier3(SB)-YPet_Blast

    Plasmid
    #169641
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and BlastR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169641-plasmid-map-sequence-id-366220

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  23. pTS2344_Tier3(SB)-mRuby2_Blast

    Plasmid
    #169642
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and BlastR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169642-plasmid-map-sequence-id-366219

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  24. pTS2339_Tier3(SB)-Ruby_Puro

    Plasmid
    #169637
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169637-plasmid-map-sequence-id-366201

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  25. pTS2354_Tier3(PB)-Ruby_PuroR

    Plasmid
    #169653
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169653-plasmid-map-sequence-id-366195

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  26. pTS2357_Tier3(PB)-TagBFP_BlastR

    Plasmid
    #169656
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mTagBFP2 and BlastR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169656-plasmid-map-sequence-id-365630

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  27. Ash1

    Plasmid
    #169711
    Purpose
    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-500
    Promoter
    T7
    Available Since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169711-plasmid-map-sequence-id-334782

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Showing: 841 - 870 of 1212 results