We narrowed to 30,195 results for: REP
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Plasmid#228831PurposeInsect cell expression of truncated BICD2 (aa 1-710)DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only
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pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRaf-IRES-pErk reporter
Plasmid#226170PurposeUsed to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.DepositorTags3xFlag-tdMCP and MycExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD80
Plasmid#223581PurposeProtein purification of human CD80 with Twin-Strep and SNAP tags from mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-PD-L2-His10
Plasmid#223578PurposeProtein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_AAVS1-OsTIR1-F74G-SNAP
Plasmid#217665PurposeRepair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locusDepositorInsertOsTir1 F74G-SNAP-Blast-AAVS1
TagsSNAPExpressionBacterial and MammalianAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-HsTtyh1-TwinStrep-His
Plasmid#211332PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)DepositorInsertTweety homology protein 1
UseLentiviralTagsTwinStrep, 10xHisExpressionMammalianMutationW417RPromoterCMVAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1-TwinStrep-His
Plasmid#210816PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)DepositorAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCi
Plasmid#214038PurposeBacterial expression plasmid for strep-tagged Pci from Haliangium ochraceumDepositorInsertPCi
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCaspase
Plasmid#214034PurposeBacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceumDepositorInsertPCaspase
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-dPCaspase
Plasmid#214035PurposeBacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceumDepositorInsertPCaspase
TagsStrep-tag IIExpressionBacterialMutationH79A, C146APromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Repo-Man-1-890
Plasmid#184272PurposeExpresses C-terminally truncated GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
TagsGFPExpressionMammalianMutationtruncated Repo-Man after aminoacid 893PromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
STREP-SUMO-FLAG-SeKaiC
Plasmid#218179PurposeWT SeKaiC for phosphorylation/dephosphorylation studyDepositorInsertSTREP-SUMO-FLAG-SeKaiC
TagsSTREP-TagExpressionBacterialAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Prepro-3x-Adan
Plasmid#181733PurposeGalactose inducible expression of Prepro-3x-AdanDepositorInsertPrepro-3x-Adan
ExpressionYeastAvailable SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
Tags6xHis and CUbo-TwinStrepExpressionBacterialAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB MBD2a-2-strep (pc1794)
Plasmid#211715PurposeExpresses fl. MBD2 2xStrep tagged in insect cellsDepositorInsertMBD2
Tags2xStrepExpressionInsectPromoterPolyhedrin PromoterAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET-C17orf53_1-87-Strep
Plasmid#211433Purposebacterial expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to a Strep-TagDepositorAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
Tags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1-PGM reporter
Plasmid#197278PurposeEncodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGMutationT15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP)
Plasmid#172339PurposemnucTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterEF1alphaAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)TetR
Plasmid#172352PurposeTet Repressor reporterDepositorInsertsTetR
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterEF1alphaAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-HsNanos1_40-56-Strep_W
Plasmid#147824PurposeBacterial Expression of HsNanos1_40-56DepositorInsertHsNanos1_40-56 (NANOS1 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-HsNanos3_4-20-Strep_W
Plasmid#147825PurposeBacterial Expression of HsNanos3_4-20DepositorInsertHsNanos3_4-20 (NANOS3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNanos3_4-20-Strep_W
Plasmid#147826PurposeBacterial Expression of HsNanos3_4-20DepositorInsertHsNanos3_4-20 (NANOS3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpG-HsNanos2_6-22-Strep_W
Plasmid#147828PurposeBacterial Expression of HsNanos2_6-55DepositorInsertHsNanos2_6-55 (NANOS2 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN23-LIC-FLAG-STREP
Plasmid#182508PurposepET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREPDepositorTypeEmpty backboneTagsSTREP-FLAG-TEVExpressionBacterialPromoterT7Available SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
TagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dR108E-cs-TEV-STREP
Plasmid#171835PurposeMammalian expression of human WIPI2d R108E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only