1,878 results

1. JH05

   Plasmid

   #73516

   Purpose

   mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::mPYet::PH::tbb-2
   

   Tags

   mYPet

   Promoter

   mex-5

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIK137

   Plasmid

   #65632

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
   

   Expression

   Worm

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLexA::nud-1

   Plasmid

   #11339

   Depositor

   Guy Caldwell

   Insert

   nud-1 (nud-1 Nematode)
   

   Tags

   LexA DNA Binding Domain

   Expression

   Yeast

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pLexA::lis-1

   Plasmid

   #11338

   Depositor

   Guy Caldwell

   Insert

   lis-1 (lis-1 Nematode)
   

   Tags

   LexA DNA Binding Domain

   Expression

   Yeast

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pEV9.03

   Plasmid

   #37495

   Depositor

   Geraldine Seydoux

   Article

   Voronina et al Development. 2010 May;137(9):1441-50. Ep

   Insert

   npp-16 (npp-16 Nematode)
   

   Use

   RNAi

   Expression

   Worm

   Mutation

   nts 162-673 of cds

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pClneoMyc' CST-1

   Plasmid

   #66859

   Purpose

   Express Myc-tagged C.elegans CST-1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   CST-1 (cst-1 Nematode)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S48N, H50R, S257P and V340A mutations in CST-1 co…

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pClneoFH-let60 G12V

   Plasmid

   #66860

   Purpose

   Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   Let-60 (let-60 Nematode)
   

   Tags

   FLAG-His6

   Expression

   Mammalian

   Mutation

   Glycine 12 is mutated to valine

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMLS285

   Plasmid

   #73734

   Purpose

   SapTrap egl-13 NLS connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   egl-13 NLS
   

   Use

   CRISPR

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSA102

   Plasmid

   #59788

   Purpose

   drives mCherry in C. elegans neurons

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   rab-3 promoter
   mCherry (worm)
   unc-54 3' UTR
   

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGC380

    Plasmid

    #19659

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*-DsRed2::let-858(3’)
    

    Use

    Flp/frt

    Expression

    Worm

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCM5.51

    Plasmid

    #19382

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    rme-2 3'utr in pDONRP2RP3
    

    Expression

    Worm

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159848

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159856

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TU#1806_CRISPR_unc-73_exon21

    Plasmid

    #82360

    Purpose

    to create unc-73E null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

    Plasmid

    #159800

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXF262

    Plasmid

    #139038

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    cox-4 (no STOP codon) (W09C5.8 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSDH50

    Plasmid

    #139037

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    tomm-20 (1-55 a.a.) (no STOP codon) (tomm-20 Nematode)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJF7

    Plasmid

    #139036

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    tomm-20 (no STOP codon) (tomm-20 Nematode)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159843

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNH078

    Plasmid

    #42164

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1
    

    Expression

    Worm

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBN263

    Plasmid

    #86729

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pmyo-2::FLP D5
    

    Expression

    Worm

    Available Since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGC180

    Plasmid

    #19616

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)
    

    Use

    Flp/frt, destination vector

    Expression

    Worm

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAS1-tax-4P::his-24::mOrange

    Plasmid

    #124340

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    tax-4, mOrange (tax-4 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAS19

    Plasmid

    #158788

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    psra-6::GCaMP6m::rpl-1a
    

    Expression

    Worm

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAS16

    Plasmid

    #158786

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Pntc-1::GCaMP6m::rpl-1a
    

    Expression

    Worm

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCZ47

    Plasmid

    #128414

    Purpose

    vab-1 rescuing minigene

    Depositor

    Andrew Chisholm

    Article

    George et al Cell. 1998 Mar 6;92(5):633-43.

    Insert

    vab-1 (vab-1 Nematode)
    

    Use

    Unspecified

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SREC 1: abu-1::gfp

    Plasmid

    #21824

    Depositor

    David Ron

    Article

    Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

    Insert

    abu-1 promoter (abu-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCM5.40

    Plasmid

    #19393

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    glp-1 3'utr in pDONRP2RP3 (glp-1 Nematode)
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGC247

    Plasmid

    #19634

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R1-R2)

    Use

    Flp/frt, destination vector

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJW1788

    Plasmid

    #154317

    Purpose

    CT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge