We narrowed to 621 results for: 1302

1. pJC45BS104 F130W

   Plasmid

   #1302

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 F130W (HSP104 Budding Yeast)
   

   Tags

   10X His tag

   Expression

   Bacterial

   Mutation

   Changed Phe 130 to Trp

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pEGFP-TDP-43 (PY-NLS)

   Plasmid

   #235490

   Purpose

   Expression of chimeric human TDP-43-EGFP bearing a PY-nuclear localization sequence (NLS) from HNRNPA1 in place of the native NLS sequence

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Replaced native NLS with PY-NLS from HNRNPA

   Available Since

   May 19, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA3-Flag-NSF/T645A

   Plasmid

   #74936

   Purpose

   Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T645A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLV.EF1α.mEmerald.CD9.miR9T

   Plasmid

   #170452

   Purpose

   Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.

   Depositor

   Tsuneya Ikezu

   Article

   Clayton et al Mol Neurodegener. 2021 Mar 22;16(1):18.

   Insert

   CD9 (CD9 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   EF1a

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

   Plasmid

   #160626

   Purpose

   TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s-dCas9:TV-Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

   Plasmid

   #160623

   Purpose

   TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:dCas9:VPR-Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Tbg-CES2A-ΔC-S227A

   Plasmid

   #200560

   Purpose

   Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
   

   Use

   AAV

   Tags

   Flag

   Promoter

   CMV promoter

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

   Plasmid

   #75400

   Purpose

   Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   hCas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEMS1097

   Plasmid

   #29028

   Purpose

   Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple201 (SLC6A5 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-Cre-NLS

   Available Since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GP804

   Plasmid

   #164548

   Purpose

   Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Smoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   All sixteen Lysines in cytoplasmic C-terminal tai…

   Promoter

   GgCryD1

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Flag-NSF/S647A

    Plasmid

    #74938

    Purpose

    Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S647A (mutation in the D2 domain of the protein)

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

    Plasmid

    #160625

    Purpose

    GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26-1gRNA-DFR 2.1
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Flag-NSF/T646A

    Plasmid

    #74937

    Purpose

    Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T646A (mutation in the D2 domain of the protein)

    Promoter

    CMV

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCSC-LHX8-IRES-GBX1

    Plasmid

    #182235

    Purpose

    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189

    Depositor

    Chun-Li Zhang

    Article

    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

    Insert

    LHX8-IRES-GBX1 (LHX8, GBX1 Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pScalps_Puro_mTet2 catalytic domain HxD

    Plasmid

    #79611

    Purpose

    Expression of catalytically inactive mouse Tet2

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet2 (Tet2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Mutation

    Mutant Tet2 with H1302Y, D1304A substitutions in …

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)

    Plasmid

    #160645

    Purpose

    Module for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLL

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsmBI sites removed

    Promoter

    35S x2

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)

    Plasmid

    #160619

    Purpose

    Module for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    ERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid

    #160621

    Purpose

    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26:gRNA 4 (pNos): 2.1 aptamer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GP805

    Plasmid

    #164545

    Purpose

    Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    All 21 cytoplamic Lysines of Smoothened changed t…

    Promoter

    GgCryD1

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFB-EF1a-DIO-Rpl22l1-3xFLAG-2A-GFP

    Plasmid

    #245377

    Purpose

    Cre-dependent expression of FLAG-tagged Rpl22l1 and EGFP.

    Depositor

    D. James Surmeier

    Article

    Geibl et al Mol Neurodegener. 2024 Oct 8;19(1):69. d

    Insert

    Rpl22l1 (Rpl22l1 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG, T2A-GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

    Plasmid

    #160628

    Purpose

    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S, 35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge