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CAG.3xHalo-GFP-SPC25

Plasmid

#176206

Purpose

Kinetochore anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

3xHalo-GFP-SPC25

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pKM061

Plasmid

#134174

Purpose

Bacterial expression of GST-HJURP

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

HJURP (HJURP Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pKM394

Plasmid

#134211

Purpose

Baculovirus expression of CENP-N and CENP-L

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N-his-CENP-L

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKM377

Plasmid

#134210

Purpose

Baculovirus expression of CENP-HKM and His-CENP-I

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-H/K/M untagged + his-I

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKM744

Plasmid

#134225

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP T + CENP W

Expression

Insect

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pKM499

Plasmid

#134216

Purpose

Baculovirus expression of CENP-U CENP-Q CENP-OP

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPU + CENPQ+ CENPOP

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKM065

Plasmid

#134175

Purpose

Retroviral plasmid expressing GFP-NLS-Plk1-PBD

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

GFP-NLS-Plk1-PBD 326-603 (PLK1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

PBD 326-603

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDK580

Plasmid

#134205

Purpose

Baculovirus expresion of CENP-L and CENP-N

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-L and His-CENP-N

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKM692

Plasmid

#134224

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 235-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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μ2-HA-WT

Plasmid

#32752

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Jip4

Plasmid

#58925

Purpose

JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.

Insert

Mapk8ip4 (Spag9 Mouse)

Tags

T7

Expression

Mammalian

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
MadMyc-HA

Plasmid

#16557

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

MADMYC (MYC Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative mutant

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pRSV-p65T305A

Plasmid

#106454

Purpose

NF-kB p65, kinase site 305 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase site 305 mutated (RELA Human)

Expression

Mammalian

Mutation

kinase site inactivated

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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G1BgSVNA-CDK4

Plasmid

#16573

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT/TO Plk4 KD-mCherry

Plasmid

#80270

Purpose

mammalian expression plasmid for kinase dead Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D154A kinase dead

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac flag Bmi1

Plasmid

#1967

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Bmi1 (Bmi1 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IL2

Plasmid

#67053

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL2 (IL2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRSV-p65S276A

Plasmid

#106455

Purpose

NF-kB p65, kinase site 276 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase site 276 mutated (RELA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

kinase site inactivated

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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