We narrowed to 747 results for: PI

1. pTE3333

   Plasmid

   #107540

   Purpose

   Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(RR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K671R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE3331

   Plasmid

   #107536

   Purpose

   Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(RVR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K613V, N617R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Endo-INPP5E

   Plasmid

   #236005

   Purpose

   Endosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

   Depositor

   Jin Zhang

   Article

   Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

   Insert

   Endo-INPP5E (INPP5E Human, Synthetic)
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM-U6-reRNA-hsRUNX

   Plasmid

   #205639

   Purpose

   reRNA guide targeting Homo sapiens RUNX

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-hsRUNX (RUNX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBD-Btk(PH)Akt1(KD)

   Plasmid

   #86591

   Purpose

   Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   PH(Btk)-KD(Akt1) fusion (AKT1, BTK Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-dCas9-FLp300

   Plasmid

   #61356

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3-Nm-dCas9-p300 Core

   Plasmid

   #61365

   Purpose

   encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D16A, D587A, H588A, N611A in Nm Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSc1-DD

   Plasmid

   #80439

   Purpose

   Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Tags

   E. coli dihydrofolate reductase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSc1-puro

    Plasmid

    #80438

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6, and hPGK

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lyso-INPP5E

    Plasmid

    #236004

    Purpose

    Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-INPP5E (LAMP1, INPP5E Human, Synthetic)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKM-U6-reRNA-mmDNMT1

    Plasmid

    #205635

    Purpose

    reRNA guide targeting Mus Musculus DNMT1

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    reRNA-mmDNMT1 (Dnmt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCdc45-mCherry

    Plasmid

    #211380

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to mCherry

    Depositor

    Helmut Pospiech

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ADH4

    Plasmid

    #38910

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ADH4 (ADH4 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pUC19_CDC45_dTAG

    Plasmid

    #232733

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Cdc45-mVen

    Plasmid

    #211390

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Cdc45-mCer

    Plasmid

    #211389

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p_sc-eVIP

    Plasmid

    #168174

    Purpose

    Expresses barcoded coding genetic variants in mammalian cells

    Depositor

    Aviv Regev, James T. Neal, Jesse Boehm

    Article

    Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905.

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    EF-1a

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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