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Addgene

We narrowed to 4,504 results for: POR C

Showing: 561 - 580 of 4504 results
  1. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pJP118

    Plasmid
    #176494
    Purpose
    Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter
    Depositor
    Insert
    Lifeact peptide
    Use
    Capsaspora owczarzaki expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. KBB106

    Plasmid
    #185065
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain
    Depositor
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup1 C-terminal region, truncated so C-terminal h…
    Promoter
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pBAD_dGFPmut3-AAV

    Plasmid
    #187379
    Purpose
    arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pNS19-LV-mutRFP-2A-GFP

    Plasmid
    #113716
    Purpose
    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)
    Depositor
    Insert
    mutRFP-2A-GFP-IRES-Puro
    Use
    Lentiviral; Reporter system
    Tags
    Expression
    Mammalian
    Mutation
    Y64L (mutRFP)
    Promoter
    Available since
    Aug. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pPPC002

    Plasmid
    #171139
    Purpose
    For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Inserts
    J1-BBa_J23117-sfGFP
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    J1-BBa_J23117 and Sp.pCas9, BBa_J23107
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pFPV25.1_dGFPmut3-LVA

    Plasmid
    #187378
    Purpose
    rpsM promoter driving the expression of destabilized GFPmut3 (containing a LVA C-terminal tail)
    Depositor
    Insert
    dGFPmut3_LVA
    Use
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    C-terminal LVA tail
    Promoter
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pAMS366-4xCDRE-GFP

    Plasmid
    #138657
    Purpose
    stable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae
    Depositor
    Insert
    4xCDRE-CYC1(PM)-yeGFP
    Use
    Yeast reporter plasmid
    Tags
    Expression
    Mutation
    Promoter
    CYC1
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 561 - 580 of 4504 results