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pET-15b-LanCL1-C322A

Plasmid

#154191

Purpose

To express LanCL1-C322A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys322->Ala322

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-MSVU-BZ

Plasmid

#160426

Purpose

Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

See depositor comments below

Promoter

AOX

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666E

Plasmid

#105313

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159E

Plasmid

#105311

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666A

Plasmid

#105309

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S666A

Plasmid

#105308

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159A

Plasmid

#105306

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159A

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-mCherry-G12V-KRAS-IRES-Blast

Plasmid

#153336

Purpose

Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

mCherry-G12V-KRAS (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pINSPECT_SigP:NLuc

Plasmid

#197263

Purpose

Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_iPE-C_P2A_Csy4

Plasmid

#232428

Purpose

Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

iPE-C–P2A–Csy4

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_CreNLS_PP7

Plasmid

#232439

Purpose

expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cre–MYC-NLS

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE8e–superNLS

Plasmid

#232429

Purpose

Expression plasmid for the ABE8e base editor with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ABE8e–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cas9–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_mGL_PP7

Plasmid

#232440

Purpose

expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

mGreenLantern

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3/FKBP12-pBirA(79-C)-ha

Plasmid

#154449

Purpose

expresses FKBP12-pBirA(79-C)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(79-C)-ha (FKBP12 Human)

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MEK1C222

Plasmid

#164638

Purpose

Bacterial expression plasmid for His6-MEK1C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S222C/C277S/C376S

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-mCherry-WT-KRAS-IRES-Blast

Plasmid

#153335

Purpose

Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

mCherry-WT-KRAS (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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TALED_Right-mouse-ATP6-1397C-AD

Plasmid

#212954

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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