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pDXinit

Plasmid

#110101

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

Depositor

Markus Seeger

Publication

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF6a-CD28-PafA

Plasmid

#113400

Purpose

Expresses CD28-PafA in mammalian cell

Depositor

Min Zhuang

Publication

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD28-PafA

Tags

myc

Expression

Mammalian

Promoter

EF-1α

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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NW50

Plasmid

#133445

Purpose

Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Publication

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW50

Expression

Bacterial

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Publication

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tetracyclin-transactivator (rtTA)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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mScarlet-EEA1

Plasmid

#169067

Purpose

Express human EEA1 in mammalian cells

Depositor

Gia Voeltz

Publication

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EEA1 (EEA1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE2 mCherry-Rtn4a

Plasmid

#86683

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Publication

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SA-betageo

Plasmid

#21709

Depositor

Philippe Soriano

Publication

Friedrich et al Genes Dev. 1991 Sep . 5(9):1513-23.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SAbgeo

Use

Mouse gene trapping

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNX32-Dest

Plasmid

#105083

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Publication

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2-TAG

Plasmid

#26772

Depositor

Shankar Srinivas

Publication

Trichas et al BMC Biol. 2008 Sep 15;6:40. doi: 10.1186

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TdTomato-2A-H2BGFP-SV40pA

Expression

Mammalian

Available since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGL2- Empty control -SV40-Luc

Plasmid

#26280

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Publication

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHAGE2 Lnp-mCherry

Plasmid

#86687

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Publication

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

LNPK (LNPK Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA5/FRT/TO-GRB2-GFP

Plasmid

#86873

Purpose

To express GRB2 with a GFP tag at C terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GRB2 (GRB2 Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GST-PRMT7

Plasmid

#34693

Depositor

Steven Clarke

Publication

Miranda et al J Biol Chem. 2004 May 28;279(22):22902-7

Insert

PRMT7 (PRMT7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcDNA3 TFAM-mClover

Plasmid

#129574

Purpose

Expression of mClover fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Publication

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Publication

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Tags

eGFP

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Flag pLJM1 Rap2A

Plasmid

#19311

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RAP2A, member of RAS oncogene family (RAP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLIB

Plasmid

#80610

Purpose

pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Promoter

polyhedrin

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pCDNA-3xHA-Reptin

Plasmid

#51636

Purpose

Mamamlian expression of 3xHA tagged reptin, N-terminal.

Depositor

Steven Artandi

Publication

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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