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ninaE-norpA

Plasmid

#26854

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

no receptor potential A (norpA Fly)

Use

Tags

Expression

Insect

Mutation

V963A

Promoter

Available since

April 5, 2011

Availability

Academic Institutions and Nonprofits only
6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Sfp

Plasmid

#159617

Purpose

Expression plasmid for Sfp phosphopantetheinyl transferase

Depositor

Jun Yin, Christopher T Walsh

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

Sfp

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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human PNP non-tagged

Plasmid

#64199

Purpose

Expression of native human PNP (non-tagged)

Depositor

Vern Schramm

Article

Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Use

Tags

none

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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HA-Cdk9

Plasmid

#28102

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

3x HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pIRES-Puro-TCPTP

Plasmid

#33366

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHJ421

Plasmid

#20680

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Use

Tags

Expression

Bacterial

Mutation

C terminal domain only

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Use

Tags

His6 and mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJ3H PTP-1B

Plasmid

#8601

Depositor

Ben Neel

Article

Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-

Insert

PTP-1B (PTPN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K103R

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Use

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBG100-TCPTP

Plasmid

#33365

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Use

Tags

6xHIS - cleavable

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pET-AspS

Plasmid

#159618

Purpose

Expression plasmid for AcpS phosphopantetheinyl transferase

Depositor

Jun Yin, Christopher T Walsh

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

AcpS

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

Plasmid

#61966

Purpose

Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-ME(pS)VD
TRAP(RK)-C-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD and PLtetO

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Dapp1-PH pET11a

Plasmid

#71376

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Dapp1-PH (DAPP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 148-273

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

Plasmid

#22930

Purpose

Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1-deltaPH (PHLPP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Includes 127-1205 aa. Deleted amino-terminal PH d…

Promoter

CMV

Available since

Feb. 3, 2010

Availability

Academic Institutions and Nonprofits only
Grp1-PH pET11a

Plasmid

#71377

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pB-puro-V5-Trpt1

Plasmid

#126607

Purpose

Expresses N-terminal V5-tagged mouse Trpt1

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

tRNA 2' phosphotransferase 1 (Trpt1 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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