We narrowed to 9,514 results for: try

1. pRP-c6MYCn

   Plasmid

   #69243

   Purpose

   Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Tags

   cMyc

   Promoter

   RRNA promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPa-iSL(MCS3+4)

   Plasmid

   #69246

   Purpose

   Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Promoter

   RRNA promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPOTv7-hyg-AtAID:HA (p5339)

   Plasmid

   #239363

   Purpose

   pPOTv7 based plasmid template for addition of HA-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

   Depositor

   Mark Carrington

   Article

   Alexandra et al bioRxiv 2025.06.17.660065

   Insert

   Arabidopsis Auxin Inducible Degradation domain
   

   Use

   Bacterial

   Tags

   1 x HA epitope

   Promoter

   none

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

   Plasmid

   #171129

   Purpose

   LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   U6 promoter entry plasmid for LwaCas13a crRNA cloning
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-G1(7FTrp)RS

   Plasmid

   #177310

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMOX-mNG::LacI-V3

   Plasmid

   #210224

   Purpose

   Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   mNeongreen Lac repressor fusion protein
   

   Use

   Trypanosoma brucei expression

   Promoter

   Pol II readthrough transcription after integration

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTrex-Neo-tdTomato

   Plasmid

   #47975

   Purpose

   Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

   Insert

   tdTomato
   

   Use

   Trypanasoma cruzi expression

   Promoter

   T. cruzi rRNA

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Promoter

   opposing T7 promoters

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-tdTomato-WPRE

   Plasmid

   #133786

   Purpose

   Cre-dependent tdTomato fluorescent reporter for cell labeling

   Depositor

   Michael Stryker

   Article

   Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

   Insert

   pAAV-EF1a-DIO-tdTomato-WPRE
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF-G1(7AW)RS

    Plasmid

    #207516

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 LC3C WT

    Plasmid

    #123078

    Purpose

    Gateway entry clone encoding human MAP1LC3C wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP1D1R1

    Plasmid

    #97323

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1R1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of R residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSP1D1G1

    Plasmid

    #97325

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1G1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of G residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223 GABARAP WT

    Plasmid

    #123080

    Purpose

    Gateway entry clone encoding human GABARAP wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP1D1T1

    Plasmid

    #97327

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1T1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of T residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 592 p3e-EST etv2 3’UTR

    Plasmid

    #49003

    Purpose

    Zebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway 3' entry

    Mutation

    only the 3' UTR

    Promoter

    3' gateway entry vector

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPOTv7-hyg-OsAID (p5110)

    Plasmid

    #239360

    Purpose

    pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Inserts

    Rice Auxin Inducible Degradation domain
    Auxin Inducible Degradation domain
    

    Use

    Bacterial

    Promoter

    none

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPOTv6-puro-3Ty:OsAID:3Ty (p5229)

    Plasmid

    #239361

    Purpose

    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Insert

    Rice Auxin inducible degradation domain
    

    Use

    Bacterial

    Tags

    3 x Ty epitope tag

    Promoter

    none

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)

    Plasmid

    #239362

    Purpose

    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Insert

    Rice Auxin inducible degradation domain
    

    Use

    Bacterial

    Tags

    3 x Ty epitope tag

    Promoter

    n/a

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGG-PIP22

    Plasmid

    #196760

    Purpose

    Contains BIK1 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    BOTRYTIS-INDUCED KINASE 1 (BIK1 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Mutation

    1878 C->G

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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