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OPEN Beta2-microglobulin

Plasmid

#215657

Purpose

E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN Beta2-microglobulin (B2M Human)

Expression

Bacterial

Mutation

Histidine 31 to Cysteine

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer

Plasmid

#174394

Purpose

Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

Depositor

Dennis Ko

Publication

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL6ST cytosolic domain with leucine zipper & C-C linker

Tags

Flag

Expression

Mammalian

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg1

Plasmid

#166998

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Publication

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 1

Use

CRISPR and Lentiviral

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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GPX8 rescue PLAK1

Plasmid

#161517

Purpose

Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

Depositor

Yoav Shaul

Publication

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

glutathione peroxidase 8 (GPX8 Human)

Use

Lentiviral

Tags

3X Flag

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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T7 promoter + rpsL reporter plasmid

Plasmid

#156455

Purpose

A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Publication

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

rpsL
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

tac and tet

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

Plasmid

#128453

Purpose

lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/intron/GFP

Plasmid

#113547

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Tags

GFP

Expression

Mammalian

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSCV-Neo-GFP/Flag-Gata5

Plasmid

#105508

Purpose

MSCV-driven retroviral Gata5 expression (Flag-tagged)

Depositor

Christopher Vakoc

Publication

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Gata5 (Gata5 Mouse)

Use

Retroviral

Tags

Flag

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFtnA-Halo

Plasmid

#98281

Purpose

mammalian expression of FtnA-Halo

Depositor

Mike Heilemann

Publication

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FtnA

Tags

Halo-tag

Expression

Mammalian

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB452

Plasmid

#74834

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB528

Plasmid

#74870

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV3

Plasmid

#78631

Purpose

GESTALT V3 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V3

Use

Lentiviral

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLJM1-EGFP-BarcodeV4

Plasmid

#78630

Purpose

GESTALT V4 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V4

Use

Lentiviral

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Publication

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Promoter

polH (polyhedrin)

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa22_4450

Plasmid

#49676

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF22_4450

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KB2

Plasmid

#23533

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KB2 (RPS6KB2 Human)

Use

Gateway Cloning

Mutation

R482Stop

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEarleyGate103 mmPing20F

Plasmid

#140007

Purpose

mmPing20F activation tag

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Plant

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDIRECT_26G

Plasmid

#91149

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-CCNE1-HA-puro

Plasmid

#212669

Purpose

Express tagged cyclin E1

Depositor

Yumi Konagaya, Tobias Meyer

Publication

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

CCNE1 (CCNE1 Human)

Use

Lentiviral

Tags

HA

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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