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pTA-attP

Plasmid

#18939

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attP

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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YIplac211-sGFP-Vrg4

Plasmid

#160904

Purpose

Expresses GFP-Vrg4 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

VRG4

Tags

GFP

Expression

Yeast

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Article

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PTPN11

Plasmid

#38965

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTPN11 (PTPN11 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
JAB2121

Plasmid

#190343

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Type

Empty backbone

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAH01SpR

Plasmid

#128159

Purpose

pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Expression

Bacterial

Promoter

Ptet

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJSM101

Plasmid

#48791

Purpose

NHEJ reporter plasmid for 5' and 3' cohesive ends

Depositor

Michael Glickman

Article

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDYU1G-TED

Plasmid

#154298

Purpose

A expression vector in Dictyostelium discoideum containing the G418 resistant gene and the TED module for GFP-negative screening

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Type

Empty backbone

Use

Dictyostelium expression

Promoter

actin15

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJSM103

Plasmid

#48872

Purpose

NHEJ reporter plasmid for blunt ends

Depositor

Michael Glickman

Article

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Ex1(-120/+3)

Plasmid

#30492

Depositor

Gerhart Ryffel

Article

Drews et al BMC Dev Biol. 2011 Jan 31;11:5. doi: 10.

Insert

lhx1 promoter

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pISH-Cbr2

Plasmid

#74339

Purpose

ISH probe for Cbr2

Depositor

Peter Mombaerts

Article

Ishii et al J Neurocytol. 2004 Dec;33(6):657-69. Epu

Insert

carbonyl reductase 2 (Cbr2 Mouse)

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only
pGST1-yVps27-VHS

Plasmid

#44417

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Vps27-VHS (2-150) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pRS314-PRP16

Plasmid

#89971

Purpose

Prp16 in a trp backbone complements prp16 genomic deletion

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

none

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGST1-yGGA2-VHS

Plasmid

#44422

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA2 (1-169) (GGA2 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yVps27-tUIM

Plasmid

#44428

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yVps27 tandem UIM (245-330) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yVps27-UIM2

Plasmid

#44427

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Vps27-UIM2 (290-330) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-STAM1-UIM

Plasmid

#44425

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

STAM1-UIM (STAM Human)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pUC-Leum

Plasmid

#44364

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

Leum promoter

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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TAL2020

Plasmid

#39378

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-11-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only