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pPOTv7-blast-BFP

Plasmid

#190258

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS546

Plasmid

#188883

Purpose

Integrating Pmex-5::Cas9 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-luxAB2

Plasmid

#27061

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pKT-SCFP3A-HIS

Plasmid

#64686

Purpose

pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

SCFP3A

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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LI dy B-C

Plasmid

#54342

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

B-C dummy

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBN266

Plasmid

#86732

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdpy-7::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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UL79-3xFLAG pCDNA4

Plasmid

#120374

Purpose

Expresses HCMV UL79 with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL79 (UL79 Human Cytomegalovirus (HHV-5))

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 nt 400-1782 delta440-500 delta1449-1735

Plasmid

#60633

Purpose

ZKSCAN1 circular RNA expression plasmid containing minimal sufficient introns

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMTG01-Amp-FRT

Plasmid

#128270

Purpose

Plasmid that produces the following fusion protein: TetR-LOV-TIP

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

TetR-LOV-TIP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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LII dy 5-6

Plasmid

#54401

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

dummy 5-6

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pNB777

Plasmid

#60153

Purpose

Red click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Expression

Yeast

Promoter

LEU1pr

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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CΔ4

Plasmid

#74299

Purpose

Mammalian expression of treacle (residues 1271-1302) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1271 - 1302

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
LI dy D-E

Plasmid

#54344

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

D-E dummy

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-EGFP-HsNanos2_6-22_X

Plasmid

#147929

Purpose

Mammalian Expression of HsNanos2_6-22

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2_6-22 (NANOS2 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTE-0X38-GCT5

Plasmid

#27051

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only
pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRiboS BsaHind

Plasmid

#36253

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBbE6a-RFP

Plasmid

#35286

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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