We narrowed to 13,360 results for: Mpl;

1. NSP6-FR-2Strep

   Plasmid

   #210317

   Purpose

   Expresses SARS-CoV-2 NSP6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Caveolin-3

   Plasmid

   #68396

   Purpose

   Fluorescently tagged Caveolin-3 for expression in mammalian cells

   Depositor

   Rob Parton

   Article

   Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

   Insert

   Cav3 (Cav3 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CaMKII-jGCaMP7f-WPRE

   Plasmid

   #203848

   Purpose

   Expression of GCaMP7f in CaMKII expressing cells

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   jGCaMP7f
   

   Use

   AAV

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR_L1-Turbo-YFP-NLS-STOP-L2

   Plasmid

   #127361

   Purpose

   gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-GFP-HS

   Plasmid

   #133305

   Purpose

   "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   hok/sok toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. bArr1-V5

   Plasmid

   #201492

   Purpose

   Expression of the beta-arrestin1 containing a C-terminal V5 tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   bArr1-V5 (ARRB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBEC2

   Plasmid

   #187596

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuRCC-K

   Plasmid

   #81193

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below., Please view…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAS9PBKS

   Plasmid

   #68371

   Purpose

   CbAkt promoter driven Cas9-2A-GFP (from px458)

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   Cas9
   

   Tags

   2A-EGFP

   Expression

   Mammalian

   Promoter

   CbAkt

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-FLEX-GT

    Plasmid

    #26198

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
    

    Use

    AAV and Cre/Lox

    Mutation

    TVA mammalian codon optimized

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPEP-TEV cNup98 (1-498)

    Plasmid

    #38037

    Depositor

    Roderick Lim

    Article

    Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

    Insert

    Nucleoporin 98 (NUP98 Human)
    

    Tags

    His-tag, 3Cysteine

    Expression

    Bacterial

    Mutation

    amino acids 1-498

    Promoter

    T7

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR660

    Plasmid

    #65216

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-8 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    P10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Puro-REDD1-HA

    Plasmid

    #133550

    Purpose

    Expresses HA tagged REDD1 in Mammalian cells

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTag-BFP-C-h-Rab5a-c-Myc

    Plasmid

    #79801

    Purpose

    Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab5a (RAB5A Human)
    

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pIDS-RESTwt full-length

    Plasmid

    #109161

    Purpose

    Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length REST/NRSF (REST Human)
    

    Expression

    Insect

    Promoter

    p10

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-N-Solo-mStayGold-TJP1

    Plasmid

    #227300

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and EF1-a

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-EF1a-Cas9GFP-W

    Plasmid

    #200100

    Purpose

    Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-GIT1

    Plasmid

    #15226

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L425V, E476K and L583P

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. mApple-6xHp

    Plasmid

    #214410

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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