We narrowed to 14,200 results for: cas9

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pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#187326

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Plasmid

#195541

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET42b-AncBE4max

Plasmid

#165157

Purpose

Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

Depositor

Taekjip Ha

Publication

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

AncBE4max

Tags

6xHis

Expression

Bacterial

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Expression

Mammalian

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAGM65881

Plasmid

#153215

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB3050(gRNA XII-5)

Plasmid

#73292

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB3020(gRNA X-2)

Plasmid

#73282

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJMR19-ADE2

Plasmid

#254119

Purpose

Candidozyma auris optimized vector expressing Cas9 and ADE2 targeting guide cassette

Depositor

Jeffrey Rybak

Publication

Doorley et al J Infect Dis. 2025 Sep 15;232(3):e529-e5

Insert

Candidozyma auris ADE2 target sequence

Use

CRISPR

Expression

Yeast

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pSEVA1213S

Plasmid

#122095

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX20

Plasmid

#169443

Purpose

Used for guide cloning with PCR

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Type

Empty backbone

Use

CRISPR

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pROS10

Plasmid

#107924

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMEL16

Plasmid

#107922

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHFS:SEP-rGluA2 TKIT donor

Plasmid

#169440

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in rat

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJAT39

Plasmid

#204289

Purpose

For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-EGFP+H3:H26

Use

CRISPR

Promoter

MHC

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSCBE3

Plasmid

#133966

Purpose

Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Publication

Zhong et al bioRxiv 630137

Insert

BE3

Use

CRISPR and Synthetic Biology

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

Plasmid

#179072

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)-Link-2xPH(PLCd)

Expression

Yeast

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179076

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-SCS2tmh

Expression

Yeast

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179073

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179062

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartment

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SNF7-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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