We narrowed to 24,925 results for: crispr
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Plasmid#138458Purposelenti vector encoding the MS2-VP64 with a 2A Hygro resistance markerDepositorPublicationInsertvp64
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU1/Sm/3' Box_(GLuc)_TOP1
Plasmid#68437PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOP1 construct
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U1Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pV1081
Plasmid#111425PurposeSolo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1DepositorPublicationInsertCaCas9/sgADE-NT2
UseCRISPRExpressionYeastAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
ExpressionBacterialPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxExpressionWormMutationHYGR∆ encodes aa 1-226Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ132A2.0
Plasmid#99887PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrp53 (Trp53 Mouse)
UseCRISPRExpressionMammalianAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable sinceSept. 14, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
px330_SMAD exon 9 gRNA
Plasmid#68352Purposepx330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMAD exon 9 gRNA
ExpressionMammalianPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY111 (pcDNA3.1-huTsCpf1)
Plasmid#92267PurposeExpression of humanized TsCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuTsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PL6-CS-WR
Plasmid#122512Purposeconstruction for sgRNA transcription with Plasmodium falciparum U6 promotorDepositorTypeEmpty backboneUseCRISPRPromoterPfU6Available sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c2
Plasmid#120873PurposeExpresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.DepositorInsertCas12c2
UseCRISPRTagsmH6ExpressionBacterialPromoterlacAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VE-cad KI gRNA1
Plasmid#92310PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVEcad KI gRNA1 (CDH5 Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKEW106-MbCas12a-58069
Plasmid#115653PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtetAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJF104B
Plasmid#113324PurposepTet-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRExpressionBacterialMutationR93AAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458_KDM1A_2
Plasmid#86298PurposeEncodes gRNA for 3' target of human KDM1ADepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA against KDM1A (KDM1A Human)
UseCRISPRAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0007
Plasmid#42247PurposegRNA targeted to zebrafish gene th1DepositorAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
EC67908
Plasmid#211795PurposeOver expresses Cas12a and GR-GRF-GIF. Must receive guide cassettesDepositorInsertHptII, Cas12a, GR-GRF-GIF
ExpressionPlantAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag
Plasmid#191537PurposeOverexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-ddMisCas13b-SNAPf-NLS-3xFlag
ExpressionMammalianAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
Tags6X His and sortase A sorting motifExpressionBacterialAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by