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pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

Plasmid

#156440

Purpose

For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA1 (Stag1 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

Plasmid

#187583

Purpose

Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

PML (PML Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

Plasmid

#182926

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET Flag TEV LIC cloning vector (1L)

Plasmid

#29662

Depositor

Scott Gradia

Type

Empty backbone

Tags

Flag and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211462

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

Plasmid

#106309

Purpose

The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)

Tags

6xflag-3xHaloTag(MtoL)

Expression

Mammalian

Promoter

EF1

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

Plasmid

#191172

Purpose

Human ADAR1, p150 isoform in plant-expressable format.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)

Tags

3FLAG and HIS

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24-exon minimal CMV pCDNA5

Plasmid

#212060

Purpose

LR vector for integration of Med24-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24-exon (Med24 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

Plasmid

#172620

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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(114) pcDNA3.1-GFP-Flag-JunB-EPEA

Plasmid

#160743

Purpose

Expresses a GFP tagged JunB with Flag and EPEA tags.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

GFP-Flag-JunB-EPEA (JUN Human)

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPdU3-MND-FLAG-FLI1-IRES-GFP

Plasmid

#129496

Purpose

Lentiviral expression of human FLI1

Depositor

Marjorie Brand

Article

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Insert

FLI1 (FLI1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

MND

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)

Plasmid

#40999

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

Plasmid

#214995

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

Plasmid

#156448

Purpose

For transient expression of human CTCF tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human CTCF (CTCF Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-LoxP-PURO-LoxP-SNAP-TERT

Plasmid

#71390

Purpose

Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Use

Hr donor

Tags

FLAG-SNAP

Promoter

Endogenous TERT promotor

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-flag-myr-Akt1-IRES-GFP

Plasmid

#65063

Purpose

mammalian retroviral expression plasmid

Depositor

Kira Gritsman, Jean Zhao

Article

Kharas et al Blood. 2010 Feb 18;115(7):1406-15. doi:

Insert

Akt1 (AKT1 Human)

Tags

flag and myristoylation tag

Expression

Mammalian

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

Plasmid

#156445

Purpose

For transient expression of mouse RAD21 (SCC1) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse RAD21 (Rad21 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF3-FLAG-IRES-GFP

Plasmid

#107388

Purpose

Lentiviral expression of IKZF3-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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