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gRNA_tdtomato_reporter_1_ST1_2x_binding

Plasmid

#79368

Purpose

Assays activity of transcriptional activators fused to ST1 Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Expression

Mammalian

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFKBPmCherry-Akt2(KD)

Plasmid

#86598

Purpose

Expresses recruitable mCherry-tagged kinase domain of human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBP-mCherry-Akt2(KD)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP8045

Plasmid

#220900

Purpose

Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

tnsABCD

Expression

Bacterial

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDonorTp

Plasmid

#211788

Purpose

pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

dhfr

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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FCK-CaMKII-Solaris-WPRE

Plasmid

#217480

Purpose

Negative response-polarity hybrid voltage indicator

Depositor

Peng Zou

Insert

Solaris

Use

Lentiviral

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Stat92E-GFP

Plasmid

#126662

Purpose

Inducible expression of Stat92E-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Stat29E

Expression

Insect

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET_scFv_QLuc

Plasmid

#183261

Purpose

Express scFv_QLuc in bacteria cells

Depositor

Huiwang Ai

Article

Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

Insert

scFv_QLuc

Tags

Histag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pZCAN

Plasmid

#129115

Purpose

Z. rouxii centromeric vector containing ClonNAT resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pECO300

Plasmid

#169766

Purpose

Express SpCas9 and Kanamycin resistance gene

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Expression

Plant

Promoter

35S, AtU6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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C163

Plasmid

#141127

Purpose

Expresses sfCFP under lac promoter

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

sfCFP

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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C164

Plasmid

#141128

Purpose

Expresses sfYFP under lac promoter

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

sfYFP

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET His10-Src42a

Plasmid

#126674

Purpose

Expression of His10-Src42a for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Src42a

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6_Pfrm_BsMdh_BmHps_BmPhi

Plasmid

#129109

Purpose

Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

Depositor

Eleftherios Terry Papoutsakis

Article

Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

Insert

BsMDH BmHPS BmPHI

Expression

Bacterial

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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CIT32u

Plasmid

#128019

Purpose

K92A

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Mutation

see Depositor Comments

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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CIT0

Plasmid

#128017

Purpose

R66A

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Mutation

see Depositor Comments

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJG486

Plasmid

#91195

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi1+donor

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A4110

Plasmid

#91056

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-dTaCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-dTaCas9-VPR

Use

CRISPR

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTaCas9_5

Plasmid

#91168

Purpose

Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab7a

Plasmid

#86638

Purpose

Expresses mCherry-tagged Rab7a

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBP-mCherry-Rab7a

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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J23112-mRFP1-331Bb

Plasmid

#78278

Purpose

mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23112-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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