We narrowed to 6,879 results for: crispr cas9 plasmids

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pICH86966::AtU6p::sgRNA:NbVPE1a/b

Plasmid

#223218

Purpose

Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

Depositor

Tsepo Tsekoa

Article

Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

Insert

NbVPE1a/b

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Plant

Promoter

Arabidopsis U6 promoter

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pPSG779

Plasmid

#239532

Purpose

CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae

Depositor

John Dueber

Insert

sgRNA_YBR197C

Use

CRISPR

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pWT019a

Plasmid

#107893

Purpose

W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pWT007f

Plasmid

#107891

Purpose

W1.0.3 plasmid. Contains PTetO-sd2U-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.3

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007a

Plasmid

#107889

Purpose

W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWT007g

Plasmid

#107890

Purpose

W1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #409 (Sa)

Plasmid

#98134

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #409

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #351 (Sa)

Plasmid

#105283

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #351

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSB700

Plasmid

#64046

Purpose

Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6, CAGGS

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA-lib 2.0

Plasmid

#89638

Purpose

sgRNA expression in mammalian cells after Gateway cloning

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDS48

Plasmid

#241839

Purpose

Cas9-gRNA construct targeting the UBE3A locus

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

Cas9-gRNA (UBE3A )

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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PEA1-Nuclease

Plasmid

#176528

Purpose

Delivers all prime editing nuclease components in a single plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Use

CRISPR

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CbH for Cas9, hU6 for gRNAs

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

pU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSL1142 (pSPIN, pBBR1 backbone)

Plasmid

#160730

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV MCS mCherry-KASH

Plasmid

#139654

Purpose

Cloning template for AAV-based ORANGE knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#206994

Purpose

Plasmid expressing both SpCas9 and SaCas9

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Insert

SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )

Tags

P2A

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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