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pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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p16 nlsGPS2

Plasmid

#228093

Purpose

Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

Depositor

Alexander Jones

Article

Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

Insert

nlsGPS2

Tags

6xHis-T7 XpressTag and cMyc

Expression

Plant

Promoter

p16

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor(R2Tg)-GFP(intron)

Plasmid

#223248

Purpose

Mammalian expression of the R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70

Plasmid

#62903

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70

Plasmid

#62902

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo1-G3BP1_pTRE-G418

Plasmid

#202015

Purpose

expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-G3BP1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsC

Plasmid

#234732

Purpose

Wild-type human-codon optimized PseTnsC in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsC

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg-3xFlag

Plasmid

#223250

Purpose

Mammalian expression of C-terminally Flag-tagged R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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hPGK-mRuby2-bGH

Plasmid

#235254

Purpose

ComMAND base gene regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLHCX-gpASNase1

Plasmid

#121526

Purpose

Expresses gpASNase1 in mammalian cells.

Depositor

Lucas Sullivan, Matthew Vander Heiden

Article

Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

Insert

gpASNase1

Use

Retroviral

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo1-NIK3x_pTRE-G418

Plasmid

#202017

Purpose

expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-NIK3x

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225063

Purpose

Expression of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pN256

Plasmid

#188472

Purpose

scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

eGFP-WPRE-DAISY-4T

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

Plasmid

#124903

Purpose

Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

p65 promoter- p65 CDS-mRuby2

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

Plasmid

#89347

Purpose

2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

Jurkat TCRalpha/beta alleles and CD3e and CD3z

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsAB

Plasmid

#234731

Purpose

Wild-type human-codon optimized PseTnsAB in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsAB

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-E504K-T2A-EGFP

Plasmid

#197037

Purpose

Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2-E504K

Use

Lentiviral

Expression

Mammalian

Mutation

E504K

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBA409

Plasmid

#85971

Purpose

EF1a-sfGFP-Ubc-Neo

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sfGFP
Neo

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRαγεζ

Plasmid

#187351

Purpose

Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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