We narrowed to 9,894 results for: UTY

1. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

   Plasmid

   #67975

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mBFP-APPP1-mGFP

   Plasmid

   #196695

   Purpose

   As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   mEGFP and mtagBFP2

   Expression

   Mammalian

   Mutation

   Lys to Val substitution at position 612

   Promoter

   CMV

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-miRFP

   Plasmid

   #108416

   Purpose

   Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   miRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3Basic-ME.2/ApoEpromoter

   Plasmid

   #51436

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   APOE apolipoprotein E (APOE, ME2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The ME.2 enhancer is fused upstream of the ApoE g…

   Promoter

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX307-mFzd5

   Plasmid

   #102867

   Purpose

   Expresses mouse Fzd5 in mammalian cells

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Frizzled 5 (Fzd5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A to G substitution at nucleotide 699 of Fzd5 ORF…

   Promoter

   EF1A

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-Archon1-KGC-EGFP-ER2

   Plasmid

   #108420

   Purpose

   AAV production plasmid encoding for Archon1 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN-hSNCA-NE

   Plasmid

   #102366

   Purpose

   Lentiviral overexpression of synuclein alpha

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Insert

   synuclein alpha (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   A silent mutation (333bp downstream of ATG) was i…

   Promoter

   EF-1a

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-iSeroSnFR-Nlgn

   Plasmid

   #128485

   Purpose

   Fluorescent reporter for serotonin (dendrite localization tag)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Has Service

   AAV9

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Full-length neuroligin, Ig-kappa leader, and cpsf…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-Archon1-KGC-GFP-ER2

   Plasmid

   #115892

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Has Service

   AAV8

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. hSyn Marina-T2A-nls-mCherry

    Plasmid

    #85843

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    GEVI Marina
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    hSyn1

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR207 SARS-CoV-2 NSP1 Delta RC

    Plasmid

    #164522

    Purpose

    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Insert

    NSP1 Delta RC (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert of NSP1 gene's CDS from SARS-CoV-2 is…

    Promoter

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3Basic_ME.1/ApoEpromoter

    Plasmid

    #51435

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    ME.1 enhancer is fused upstream of ApoE promoter …

    Promoter

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK2045-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(CMV-gRNA1)

    Plasmid

    #223165

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting CMV

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2

    Plasmid

    #108422

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Has Service

    AAV5

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmiRFP-alpha-Tubulin-19-C

    Plasmid

    #108413

    Purpose

    Alpha-tubulin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    alpha-tubulin b (Tuba1b Mouse)
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-TRC.mKO2_shB3GNT5.1

    Plasmid

    #110325

    Purpose

    TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    B3GNT5 (B3GNT5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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