We narrowed to 81,735 results for: TRI
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Plasmid#148360PurposeBacterial Expression of DmMextli_577-640DepositorInsertDmMextli_577-640 (mxt Fly)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-CeeIF4E3_1-215opt-V58AI74A_AB
Plasmid#148368PurposeBacterial Expression of CeeIF4E3_1-215-V58AI74ADepositorInsertCeeIF4E3_1-215-V58AI74A
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiT-mEmerald-MED1 donor
Plasmid#197867PurposeDonor plasmid used for building mEmerald-MED1 knock-in mESCsDepositorAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-mNG
Plasmid#195160Purposemammalian expression of siRNA-resistant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Stim1NTD-ABHD16Acyto-mNG
Plasmid#195158Purposemammalian expression of ER localized ABHD16A tagged with mNeonGreenDepositorInsertStim1NTD (STIM1 Human)
TagsABHD16A cytoplasmic domain and mNeonGreenExpressionMammalianMutationNonePromoterCMVAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UIM-mut
Plasmid#187147PurposeEntry cloneDepositorAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_F3194S
Plasmid#187140Purposemammalian cell expression of FLAG-HUWE1 F3194SDepositorAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_3xFLAG-HUWE1_H669Q
Plasmid#187138Purposemammalian cell expression of 3xFLAG-HUWE1 H669QDepositorAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-HUWE1-HECTonly
Plasmid#187119Purposeinsect cell expression of HUWE1 HECT domainDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM-mut
Plasmid#187151PurposeEntry cloneDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBM-mut
Plasmid#187149PurposeEntry cloneDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA-mut
Plasmid#187145PurposeEntry cloneDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_BH3-4E
Plasmid#187143PurposeEntry cloneDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_delta169-189
Plasmid#187134PurposeEntry cloneDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_delta3896-3951
Plasmid#187132PurposeEntry cloneDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_I3969A/F3982A
Plasmid#187128PurposeEntry cloneDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_YH/GG_delta3291-3298
Plasmid#187126PurposeEntry cloneDepositorAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_delta3291-3298
Plasmid#187124PurposeEntry cloneDepositorAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_YH/GG
Plasmid#187122PurposeEntry cloneDepositorAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0751
Plasmid#185628PurposeModified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaIDepositorInsertlacZ
UseCRISPR and Synthetic BiologyAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y
Plasmid#148075PurposeInsect Expression of DmPAN2-F1103A-dsRNAresDepositorInsertDmPAN2-F1103A-dsRNAres (PAN2 Fly)
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y
Plasmid#148046PurposeBacterial Expression of DmPAN2-K1124EY1127EK1131E-dsRNAresDepositorInsertDmPAN2-K1124EY1127EK1131E-dsRNAres (PAN2 Fly)
ExpressionBacterialAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TMD18
Plasmid#163673PurposeExpresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD19
Plasmid#163674PurposeExpresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-ST*-GFP
Plasmid#163669PurposeExpresses FKBP tagged partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
TagsFK506 binding protein (FKBP) and GFPExpressionMammalianMutationThis chimera contains the first 113 amino acids o…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2-IR2 GFP pZa
Plasmid#170083PurposeGFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPdnaK-IR2-IR2Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e1 sgRNA / hSpCas9
Plasmid#172830PurposeMammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e2 sgRNA / hSpCas9
Plasmid#172831PurposeMammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Camk2a sgRNA / hSpCas9
Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin-FLN-ELP-His-ybbr
Plasmid#168039PurposeExpresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
Tags3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1-5p
Plasmid#169712PurposeBacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removedDepositorInsertAsh1 (ASH1 Budding Yeast)
Tags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-480…PromoterT7Available SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH2 #7
Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE42
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
ExpressionYeastPromoterTEF1Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XXL)Cas9
Plasmid#127387PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XXL)-Cas9
UseCRISPRExpressionInsectAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-Dronpa
Plasmid#138250PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-Dronpa
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only