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pFA6a-FLAG-APEX2-NES-NatMX6

Plasmid

#229231

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP DEPP1-mCherry

Plasmid

#224559

Purpose

Expresses DEPP1 fused to mCherry in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

DEPP1 (DEPP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP DEPP1 dTSnare-mCherry

Plasmid

#224561

Purpose

Expresses DEPP1 dTsnare fused to mCherry in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

DEPP1 (DEPP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

removed Tsnare domain

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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ECMAB - pET22b Ag43 160N 6H Mms6

Plasmid

#225539

Purpose

Construction of extracellular magnetite accumulating bacterial systems.

Depositor

Urartu Seker

Article

Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

Inserts

Iron binding protein
Autotransporter protein

Tags

no

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

Plasmid

#231592

Purpose

Express circular RNA translation reporter with 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015898

Plasmid

#226285

Purpose

Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1
Eugenol O-methyltransferase

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Plasmid

#217970

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

Depositor

Robert Piper

Article

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-min5UTR-hRluc-A60

Plasmid

#182638

Purpose

Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase,

Use

Luciferase

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT011-ZF6 1x FLAG-synTF-PPF

Plasmid

#202501

Purpose

Express "PPF" synTF (ZF6)

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

p65-ZF6

Tags

1x FLAG and Palmitoylation-Palmitoylation-Farnesy…

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSMART-Ala2

Plasmid

#71326

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

insulated waaHp from E. coli

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot-C-BIOT

Plasmid

#140186

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Biotinylation and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 gRNA17 (FWA)

Plasmid

#120250

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-mCherry-FHA1-subT391A-mCherry

Plasmid

#104462

Purpose

Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

Depositor

Bernardo Sabatini

Article

Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

Insert

mCherry-subT391A-FHA1-mCherry

Use

AAV

Tags

mCherry

Promoter

CAG

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCas59

Plasmid

#82396

Purpose

sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAN-OR-MalT-3NT

Plasmid

#62309

Purpose

synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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