We narrowed to 29,895 results for: des.2

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pHR-SNAP-CD86iso2

Plasmid

#223574

Purpose

Lentiviral expression of human CD86 isoform 2 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 WT

Plasmid

#185632

Purpose

expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 W157E

Plasmid

#185641

Purpose

expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

W157E

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 F158E

Plasmid

#185642

Purpose

expression of GST-tagged mutant PML B-box1 F158E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

F158E

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-RB_B

Plasmid

#145977

Purpose

Insect Expression of DmAGO1-RB

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmAGO1-RB (AGO1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Tags

Strep Sumo

Expression

Insect

Promoter

polyhedrin

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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p1.1-Tr2-eGFP

Plasmid

#162782

Purpose

Fluorescent reporter for protein expression studies

Depositor

Ivan Vorobiev

Article

Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

Insert

enhanced green fluorescent protein

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-Bik-pEGFP-C1

Plasmid

#166737

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bik (BIK Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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CRY-tTA (B702)

Plasmid

#92032

Purpose

Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

tTA2 (TetR fused to VP64)

Expression

Mammalian

Mutation

NLS on CRY2 is mutated

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJK2097

Plasmid

#218665

Purpose

Expression of MBP:6xHIS:FBF-2(Y479A, 121-632)

Depositor

Judith Kimble

Article

Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

Insert

fbf-2 (fbf-2 Nematode)

Tags

MBP-6xHIS

Expression

Bacterial

Mutation

amino acids 121-632 only, with Y479A mutation

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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cacna2d1 BBS pMT2

Plasmid

#206062

Purpose

expression of rat calcium channel alpha2delta-1 with a single Bungarotoxin Binding Site motif inserted after aa549

Depositor

Annette Dolphin

Article

Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16 (B695)

Plasmid

#92031

Purpose

Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

GalBD-VP16AD

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00167

Plasmid

#34320

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12399 (Mad Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Flag-HA-USP2

Plasmid

#22577

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP2 (USP2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP15

Plasmid

#22570

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP15 (USP15 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUB2

Plasmid

#22552

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUB2 (OTUB2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2M1

Plasmid

#202758

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2M1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgPTEN

Plasmid

#202759

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting PTEN

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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