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pAAV-Tnnt2-HA-Luciferase

Plasmid

#227803

Purpose

cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of luciferase and a HA tag

Depositor

Yuxuan Guo

Article

Chen et al Fundam Res. 2025 Jun 30. doi: https://do

Insert

HA-Luciferase

Use

AAV

Expression

Mammalian

Promoter

Tnnt2

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaq

Plasmid

#236750

Purpose

Express HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

Insert

Gnaq (Gnaq Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

Plasmid

#200877

Purpose

ZooTsy_TnsF expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_TnsF

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT384: pSC101-Donor(ZooTsy)

Plasmid

#200875

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT81: pTarget(ZooTsy)

Plasmid

#200876

Purpose

Target plasmid containing attachement site for ZooTsy.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_comM

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT315: pHelper(ZooTsy)_TnsF-Y584F

Plasmid

#200872

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT375: pDonor(ZooTsy)_excision-reporter

Plasmid

#200873

Purpose

Reporter for ZooTsy excision.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT155: pHelper(ZooTsy)_ΔHTH

Plasmid

#200868

Purpose

Inducible expression of ZooTsy (except HTH) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT156: pHelper(ZooTsy)_ΔTnsF

Plasmid

#200869

Purpose

Inducible expression of ZooTsy (except TnsF) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT148: pHelper(ZooTsy)

Plasmid

#200866

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT154: pHelper(ZooTsy)_ΔYRec

Plasmid

#200867

Purpose

Inducible expression of ZooTsy (except YRec) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT368: pSC101-Donor(AjTn6022)

Plasmid

#200864

Purpose

AjTn6022 donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT112: pTarget(AjTn6022)_U50D50

Plasmid

#200862

Purpose

Target plasmid containing attachement site for AjTn6022.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_comM

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT91: pR6K-Donor(AjTn6022)

Plasmid

#200863

Purpose

AjTn6022 donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT385: pHelper(AjTn6022)_QF-separated

Plasmid

#200859

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT147: pHelper(AjTn6022)

Plasmid

#200851

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK83

Plasmid

#226793

Purpose

Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Insert

slr0214 (slr0214 Synechocystis sp. PCC 6803)

Expression

Bacterial

Promoter

Tetracycline resistance cassette promoter (consti…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pACYC-yhaM

Plasmid

#207650

Purpose

Overexpression of native L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5c-cdl

Plasmid

#207649

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Tags

6xHis

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-cdl

Plasmid

#207652

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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