We narrowed to 8,515 results for: Set

1. BtuF

   Plasmid

   #92209

   Purpose

   Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

   Depositor

   Douglas Rees

   Article

   Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

   Insert

   BtuF (btuF )
   

   Use

   Tags

   6x His Tag and PelB Signal Sequence

   Expression

   Bacterial

   Mutation

   BtuF signal sequence removed and uses the PelB si…

   Promoter

   T7 Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_B2112

   Plasmid

   #91064

   Purpose

   Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   PvUbi1

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

   Plasmid

   #211904

   Purpose

   Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   CD19-CAR-28z-2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS44i8GM

   Plasmid

   #207527

   Purpose

   Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G2_Dual_sgRNA

   Plasmid

   #173201

   Purpose

   Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_B2203

   Plasmid

   #91066

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. JN302: pMVP/SB/Puro-DEST

   Plasmid

   #121865

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSIV-TRE-ASCL1

   Plasmid

   #154883

   Purpose

   Doxycycline-dependently expresses human ASCL1.

   Depositor

   Hideyuki Okano

   Article

   Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

   Insert

   ASCL1 (ASCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2103b

   Plasmid

   #91062

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78304

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADfluw-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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