We narrowed to 14,435 results for: NTS

1. pET28C-mCherry-YTHDF2

   Plasmid

   #204406

   Purpose

   E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIP-GFP-STX17ΔNTD

   Plasmid

   #89941

   Purpose

   Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

   Insert

   human syntaxin17 lacking N-terminal domain (STX17 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   deleted amino acids 1-161

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGM65905

   Plasmid

   #153217

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-NPY-ClopHensor

   Plasmid

   #25939

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
   

   Tags

   NPY and Strep-II

   Expression

   Mammalian

   Mutation

   Neuropeptide Y localization signal

   Available Since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT9658-sgRNA-mCherry

   Plasmid

   #162987

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_C2906

   Plasmid

   #91093

   Purpose

   Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TREX2 (+ BaeI site for GT donor cloning) (Trex2 Mouse)
   

   Use

   Unspecified

   Promoter

   FMV 34S

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB521

   Plasmid

   #74863

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. NMD2_lenti

   Plasmid

   #216221

   Purpose

   Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns in lenti backbone

   Depositor

   Rebecca Voorhees

   Article

   Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

   Insert

   GFP P2A RFP_b-globin intron x2
   

   Expression

   Mammalian

   Available Since

   April 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT9650-BEAR-GFP-preedited

   Plasmid

   #162992

   Purpose

   BEAR control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MKK2

    Plasmid

    #29580

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    MKK2 (MAP2K2 Human)
    

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Constitutively active mutant; Starting Met remove…

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pGWB520

    Plasmid

    #74862

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB410

    Plasmid

    #74804

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLP-pTac-Tn5-chlorR

    Plasmid

    #199994

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_085

    Plasmid

    #158583

    Purpose

    Lentiviral expression of SpCas9; P2A site provides mKate2

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Insert

    SpCas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE-30-RexYFP

    Plasmid

    #141070

    Purpose

    Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    RexYFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed Leucine 169 to Proline, changed Tyrosine …

    Promoter

    T5

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO-YHRC

    Plasmid

    #54336

    Purpose

    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

    Depositor

    Claes Andréasson

    Article

    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

    Type

    Empty backbone

    Tags

    6His-SUMO (yeast gene: SMT3)

    Expression

    Bacterial

    Promoter

    T7-LacO

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_human-pro-IL-18_DeltaN24

    Plasmid

    #214327

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Deletion of first 24 amino acids

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac_human-pro-IL-18

    Plasmid

    #214337

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGM55297

    Plasmid

    #153213

    Purpose

    Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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