We narrowed to 15,073 results for: NTS

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pU6-1 (GB1204)

Plasmid

#75405

Purpose

Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

AtU6-1 promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A24-VN

Plasmid

#135508

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*24:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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373_ESR1 in pmiRGlo

Plasmid

#78134

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

ESR1 (ESR1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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B-SpCas9

Plasmid

#126760

Purpose

Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRLCon1-1746

Plasmid

#31446

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR (short) (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1-VN

Plasmid

#135502

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*01:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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BRCA1-(219-758)

Plasmid

#35081

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 219-758

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(663-894)

Plasmid

#35072

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 663-894

Promoter

T7

Available Since

May 14, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(758-936)

Plasmid

#35073

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 758-936

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(408-540)

Plasmid

#35069

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 408-540

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
FSW-HRP-V5-MDGA2

Plasmid

#82542

Purpose

Lentiviral vector for expressing HRP-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA1-(1443-1649)

Plasmid

#35080

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1443-1649

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00664

Plasmid

#39719

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18023 (Eip78C Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pX61

Plasmid

#46852

Purpose

TOP Hupki-puro2A-Hupki-wt plasmid

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 wild type (TP53 Human, Mouse)

Expression

Mammalian

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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KHBD00675

Plasmid

#39723

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6883 (trh Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pRR-S1

Plasmid

#69998

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G329T (G110V)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S2

Plasmid

#69999

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

T441C (I114T)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S4

Plasmid

#70000

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G835C (A279P)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S5

Plasmid

#70001

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

T548G (V183G)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S6a

Plasmid

#70002

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

T637C (F213L)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S7

Plasmid

#70003

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G758A (R253H)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-S8

Plasmid

#70004

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

C1459A (R487S)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pIKAT127

Plasmid

#70005

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G444T (E148D)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-D4J3S7

Plasmid

#103096

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

D4J3S7(Cas9 coding gene from Coprococcus catus GD/7)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00652

Plasmid

#39713

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11799 (fd68A Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pTC362

Plasmid

#91216

Purpose

protoplast vector for targeted deletion of 6 genes in tomato

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting 6 tomato genes

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-E3HCA8

Plasmid

#103100

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

E3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-A8REH9

Plasmid

#103084

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

A8REH9(Cas9 coding gene from Eubacterium dolichum DSM 3991)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-J6LE60

Plasmid

#103117

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

J6LE60(Cas9 coding gene from Rhodovulum sp. PH10)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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