We narrowed to 9,135 results for: mel

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pET28b_H6-Smt3-AesC-GFP

Plasmid

#234486

Purpose

Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

C-terminal Fragment of the Aes123 PolB1 Split Intein

Tags

Hexahistidine Tag, Superfolder Green Fluorescent …

Expression

Bacterial

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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F-MED26

Plasmid

#15367

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED26 (MED26 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
ABE8.20-d

Plasmid

#136301

Purpose

expresses ABE8.20-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINTphiC31

Plasmid

#127518

Purpose

Plasmid encodes H. sapiens codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Plasmid

#217510

Purpose

KD Recombinase dependent conditional smGdP-10XV5 cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Tags

smGdP-10XV5

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGAPDH::H2B-mScarlet

Plasmid

#186764

Purpose

A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

H2B-mScarlet

Use

Overexpression

Tags

H2B

Promoter

GAPDH

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-puro TPR-Met

Plasmid

#10902

Depositor

Bob Weinberg

Article

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

TPR-Met (MET, TPR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Constitutively active form of the c-Met receptor

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pSECC

Plasmid

#60820

Purpose

3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

Depositor

Tyler Jacks

Article

Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

Inserts

Cas9
Cre

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Mammalian

Mutation

BsmBI site eliminated by C->A; E308G

Promoter

EFS and EFS (after Cas9-2A)

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-ARF

Plasmid

#89123

Purpose

mammalian expression of p14-hARF with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

Insert

p14-hARF (CDKN2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET-NlaCascade-NLS-6xHis

Plasmid

#180213

Purpose

Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas5-Cas8-Cas7

Tags

6XHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB1-mCherry-6xHis

Plasmid

#130984

Purpose

Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-mCherry-6xHis (Mapre1 Mouse)

Tags

mCherry-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p5E--3mnx1

Plasmid

#74632

Purpose

Can be used to drive expression specifically in zebrafish motor neurons

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO-2TO

Plasmid

#86697

Purpose

Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 2TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shQKI-3373

Plasmid

#115456

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3373 (QKI Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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ABE8.17-m

Plasmid

#136298

Purpose

expresses ABE8.17-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.17-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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