We narrowed to 15,932 results for: nans

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pQE80L SpyTag-ELP-SpyTag DK

Plasmid

#112635

Purpose

SpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DK

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

SpyTag-ELP-SpyTag DK

Tags

His6

Expression

Bacterial

Mutation

Contains central cysteine. Reactive aspartic acid…

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSECB

Plasmid

#106305

Purpose

Express spCas9 with sgRNA, blast resistance

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2_BirA

Plasmid

#136521

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Entry vector for gateway cloning

Mutation

R118G (highly promiscuous form)

Available Since

June 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAB029f

Plasmid

#79220

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-SH2ABL1; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAB031a

Plasmid

#79221

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-Zif268; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKM53

Plasmid

#154156

Purpose

Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel1A NF-κB site

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Promoter

8 copies of the Aedes Aegypti Rel1A NF-κB promoter

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pONL-N1_LAMP1

Plasmid

#65686

Purpose

Expresses LAMP1-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pONL-C1_H2B

Plasmid

#65704

Purpose

Expresses ONL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-Cx26-7

Plasmid

#55473

Purpose

Localization: Gap Junctions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Cx26

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Profilin-10

Plasmid

#55506

Purpose

Localization: Cytoskeleton, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Profilin

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
AAV-CMV-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159962

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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SEPLuc

Plasmid

#141102

Purpose

bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc

Use

Lentiviral

Promoter

EF1α

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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SERA6-bio-His

Plasmid

#50818

Purpose

Expresses enzymatically monobiotinylated full length SERA6

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA6

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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mNG-mCh

Plasmid

#124215

Purpose

Cytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mCh

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

mNG-mCh

Expression

Bacterial

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCS1

Plasmid

#55749

Purpose

Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Promoter

T7-lac

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-210-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85920

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-210-3p Sensor (MIR210 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-325-Decoy.hPGK.GFP.WPRE

Plasmid

#46610

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-325 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only