We narrowed to 8,358 results for: BLI

1. pLEX307-CTSB-puro

   Plasmid

   #158463

   Purpose

   Constitutive lentiviral expression of human CTSB.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   CTSB (CTSB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT-TODestGFP_HuD

   Plasmid

   #65763

   Purpose

   expresses GFP-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. RHPMP132 (mEOS3.1-beta Actin)

   Plasmid

   #88836

   Purpose

   Localization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1

   Depositor

   Ricardo Henriques

   Insert

   beta-Actin (ACTB Human)
   

   Use

   Tags

   mEOS3.1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-Puro PRMT5-CRISPRi

   Plasmid

   #164637

   Purpose

   lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

   Depositor

   Günter Schneider

   Insert

   target guide sequence PRMT5 inhibition (PRMT5 Human)
   

   Use

   CRISPR and Lentiviral; Interference

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1-CII

   Plasmid

   #51566

   Purpose

   Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

   Depositor

   Sankar Adhya, Francisco Malagon

   Insert

   cII
   

   Use

   Tags

   Intein-Chiting Binding Domanin

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. RHPMP133 (mOrange2-beta Actin)

   Plasmid

   #88837

   Purpose

   Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2

   Depositor

   Ricardo Henriques

   Insert

   beta-Actin (ACTB Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hACE2-cMYC-Flag

   Plasmid

   #158957

   Purpose

   Used for expression of human ACE2 with AAV packaging

   Depositor

   Akiko Iwasaki

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   AAV

   Tags

   Flag and MYC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. XLone-BSD SARS-CoV2 N P2A mCherry

   Plasmid

   #154398

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SARS-CoV2 N Protein (N SARS-CoV2)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX307-FURIN-G418

   Plasmid

   #158462

   Purpose

   Constitutive lentiviral expression of human FURIN.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   FURIN (FURIN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-Kras S17N

    Plasmid

    #58899

    Purpose

    Expresses human Kras with a S17N mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S17N mutation

    Promoter

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid

    #80077

    Purpose

    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

    Depositor

    Phillip Abbosh

    Inserts

    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    

    Use

    Retroviral

    Tags

    5' FLAG

    Expression

    Mammalian

    Mutation

    Y641F, R342R, N393N

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ApaLI*-HA

    Plasmid

    #211461

    Purpose

    Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI mutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D211A, E225A, K227A

    Promoter

    CMV

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRT-DestFLAGHA_HuB

    Plasmid

    #65758

    Purpose

    constitutively expresses FLAG-HA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

14. AURKB

    Plasmid

    #39119

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pDONR221-APN

    Plasmid

    #158440

    Purpose

    Gateway Cloning compatible entry vector for the human APN gene.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT-HA-STX1

    Plasmid

    #89310

    Purpose

    Expresses HA tagged syntaxin 1 in Drosophila cells

    Depositor

    Andrew Peden

    Insert

    STX1 (Syx1A Fly)
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Promoter

    P-MT (Metallothionein)

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT-TODestGFP_HuB

    Plasmid

    #65761

    Purpose

    expresses GFP-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Hs.NRAS Q61R

    Plasmid

    #83179

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61R

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. BASIC_SEVA_17_pKD46.10

    Plasmid

    #178917

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pRN3P_TIR1

    Plasmid

    #119766

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    TIR1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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