We narrowed to 16,387 results for: LEA

Showing: 5921 - 5940 of 16387 results

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GFP-WT (AF41)

Plasmid

#21367

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

Plasmid

#122083

Purpose

Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-LaminB1

Use

Retroviral

Tags

V5 tag on Nter of LaminB1

Expression

Bacterial and Mammalian

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Structural polyprotein

Plasmid

#215698

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-TEV-TBP6.9F34MF37MF77M

Plasmid

#168268

Purpose

Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

TBP6.9

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F34M, F37M and F77M

Promoter

T7 promotor

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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AV15_pCAG.Cas9.gRNA.S1

Plasmid

#199259

Purpose

Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR1-660

Plasmid

#89079

Purpose

mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids

Depositor

Elizabeth Wilson

Article

Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

Insert

hAR-1-660 (AR Human)

Expression

Mammalian

Mutation

aa 1-660 (contains exons A,B,C and part of D)

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Miwi-EGFP

Plasmid

#212575

Purpose

Lentiviral expression of Flag-tagged mouse Miwi in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Miwi (Piwil1 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-VMAT2-WPRE-pA

Plasmid

#39339

Depositor

Bernardo Sabatini

Article

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV; R

Mutation

Myc tag inserted in the first lumenal loop betwee…

Available Since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pX330 Human 3' HP1a gRNA

Plasmid

#127907

Purpose

WT Cas9 Vector targeting the 3' end of the human HP1a gene

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

gRNA for Human 3' HP1a

Use

CRISPR

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.3

Plasmid

#190797

Purpose

aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT2h

Plasmid

#208721

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

Plasmid

#228722

Purpose

Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2702-CMV-iNme2-ABE8e-nt

Plasmid

#228723

Purpose

Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with iNme2 nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2703-CMV-iNme2Smu-ABE8e-nt

Plasmid

#228724

Purpose

Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with iNme2Smu nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-G2-ATP8-V5 (puromycin)

Plasmid

#86852

Purpose

mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Tags

ATP5G2 (G2) mitochondrial targeting signal and V5

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

Plasmid

#226011

Purpose

CasRx guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Article

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Promoter

ptac

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_3_dCas9_Mxi1_sgRNA

Plasmid

#163708

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
Mxi1+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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