We narrowed to 17,821 results for: Por
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Plasmid#148103PurposeBacterial Expression of NcPAN2_1-390DepositorInsertNcPAN2_1-390
ExpressionBacterialAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-mEmerald-ER-5
Plasmid#170715PurposeExpresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.DepositorInsertAPV-DsRed2-mEmerald-ER-5
ExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-FLAG-DsRed2-ER-5
Plasmid#170620PurposeExpresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.DepositorInsertAPV-FLAG-DsRed2-ER-5
ExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_APV-SBP-Dendra2-ER-5
Plasmid#170679PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.DepositorInsertsAPV-SBP-Dendra2-ER-5
streptavidin-KDEL
ExpressionMammalianPromoterIRES and T7Available SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-mEmerald-DsRed2-ER-5
Plasmid#170681PurposeExpresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.DepositorInsertAPV-mEmerald-DsRed2-ER-5
ExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS105
Plasmid#174830PurposeEncodes a unique yeast telomere end at TEL11R by removing the subtelomereDepositorInsert11R Unique
TagsGAL4 5XUASExpressionBacterialMutationChanging TEL11R to a unique endPromoterlac promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC031
Plasmid#171152PurposepGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassetteDepositorInsertJ3-BBa_J23117-mRFP
ExpressionBacterialPromoterJ3-BBa_J23117Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC034
Plasmid#171153PurposepGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassetteDepositorInsertJ3(106)- BBa_J23111-sfGFP
ExpressionBacterialPromoterJ3(106)-BBa_J23111Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-sfGFP-pA-FRT-Amp-FRT
Plasmid#176620Purposefor generating PCR templates with sfGFP and Amp for BAC recombinationDepositorInsertsuper folder green fluorescent protein with FRT-flanked ampicillin cassette
UseUnspecifiedMutationH217R - please see dep. commentsAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
NmMetNI-WT
Plasmid#172114PurposeExpression of MetN and MetI membrane proteinsDepositorInsertsMetN
MetI
Tags10x His Tag and EnterokinaseExpressionBacterialPromoterT7 PromoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC016.109
Plasmid#171144PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC008.106
Plasmid#171141PurposeExpression of J106 scRNA on pBBR1-GmR plasmidDepositorInsertJ106 scRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MPC021: CamKII CMV_sNovArch_citrine (soma targeting)
Plasmid#153194Purposeencoded soma targeted NovArchDepositorInsertsNovArch
TagscitrineExpressionMammalianAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOCT4-Cyan-pac
Plasmid#160871PurposeMarmoset TargetingDepositorInsertmCerulean3
UseMarmoset targetingAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc120
Plasmid#161950PurposeContains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmidDepositorInsertpSB4A3-PECF20-B30-mScarlet-I-B15
ExpressionBacterialPromoterPECF20Available SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T4
Plasmid#161485PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T4_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (targeting T4 of pTHI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T5
Plasmid#161486PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (targeting T5 of pTHI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T2
Plasmid#161489PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T2_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T2 in the THI11 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only